K-ras gene mutation in colorectal adenomas and carcinomas from familial adenomatous polyposis patients.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108876
Title:
K-ras gene mutation in colorectal adenomas and carcinomas from familial adenomatous polyposis patients.
Authors:
Boughdady, I S; Kinsella, Anne R; Haboubi, N Y; Schofield, Philip F
Abstract:
Colorectal adenomas and carcinomas from familial adenomatous polyposis (FAP) patients were screened for the presence of K-ras gene mutations at codon 12 using an in vitro amplification step (polymerase chain reaction) followed by dot blot analysis using oligonucleotide probes specific for different mutations at codon 12. We examined 28 colorectal adenomas and two colorectal carcinomas from 12 FAP patients and observed a mutation at codon 12 in seven adenomas and in both carcinomas. The frequency of K-ras gene mutations in colorectal tumours from FAP patients is similar to those in cases of sporadic adenomas and sporadic colorectal carcinomas indicating that the mechanisms involved in their development may be similar.
Affiliation:
Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
Citation:
K-ras gene mutation in colorectal adenomas and carcinomas from familial adenomatous polyposis patients. 1992, 1 (4):269-74 Surg Oncol
Journal:
Surgical Oncology
Issue Date:
Aug-1992
URI:
http://hdl.handle.net/10541/108876
DOI:
10.1016/0960-7404(92)90087-2
PubMed ID:
1341260
Type:
Article
Language:
en
ISSN:
0960-7404
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBoughdady, I Sen
dc.contributor.authorKinsella, Anne Ren
dc.contributor.authorHaboubi, N Yen
dc.contributor.authorSchofield, Philip Fen
dc.date.accessioned2010-08-03T08:22:08Z-
dc.date.available2010-08-03T08:22:08Z-
dc.date.issued1992-08-
dc.identifier.citationK-ras gene mutation in colorectal adenomas and carcinomas from familial adenomatous polyposis patients. 1992, 1 (4):269-74 Surg Oncolen
dc.identifier.issn0960-7404-
dc.identifier.pmid1341260-
dc.identifier.doi10.1016/0960-7404(92)90087-2-
dc.identifier.urihttp://hdl.handle.net/10541/108876-
dc.description.abstractColorectal adenomas and carcinomas from familial adenomatous polyposis (FAP) patients were screened for the presence of K-ras gene mutations at codon 12 using an in vitro amplification step (polymerase chain reaction) followed by dot blot analysis using oligonucleotide probes specific for different mutations at codon 12. We examined 28 colorectal adenomas and two colorectal carcinomas from 12 FAP patients and observed a mutation at codon 12 in seven adenomas and in both carcinomas. The frequency of K-ras gene mutations in colorectal tumours from FAP patients is similar to those in cases of sporadic adenomas and sporadic colorectal carcinomas indicating that the mechanisms involved in their development may be similar.en
dc.language.isoenen
dc.subjectColorectal Canceren
dc.subject.meshAdenoma-
dc.subject.meshAdenomatous Polyposis Coli-
dc.subject.meshAdult-
dc.subject.meshBase Sequence-
dc.subject.meshCarcinoma-
dc.subject.meshChild-
dc.subject.meshCodon-
dc.subject.meshColorectal Neoplasms-
dc.subject.meshDNA-
dc.subject.meshFemale-
dc.subject.meshGenes, ras-
dc.subject.meshHumans-
dc.subject.meshImmunoblotting-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMutation-
dc.subject.meshNucleic Acid Hybridization-
dc.subject.meshOligonucleotide Probes-
dc.subject.meshPolymerase Chain Reaction-
dc.titleK-ras gene mutation in colorectal adenomas and carcinomas from familial adenomatous polyposis patients.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalSurgical Oncologyen

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