K-ras gene mutation in colorectal adenomas and carcinomas from familial adenomatous polyposis patients.
Affiliation
Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.Issue Date
1992-08
Metadata
Show full item recordAbstract
Colorectal adenomas and carcinomas from familial adenomatous polyposis (FAP) patients were screened for the presence of K-ras gene mutations at codon 12 using an in vitro amplification step (polymerase chain reaction) followed by dot blot analysis using oligonucleotide probes specific for different mutations at codon 12. We examined 28 colorectal adenomas and two colorectal carcinomas from 12 FAP patients and observed a mutation at codon 12 in seven adenomas and in both carcinomas. The frequency of K-ras gene mutations in colorectal tumours from FAP patients is similar to those in cases of sporadic adenomas and sporadic colorectal carcinomas indicating that the mechanisms involved in their development may be similar.Citation
K-ras gene mutation in colorectal adenomas and carcinomas from familial adenomatous polyposis patients. 1992, 1 (4):269-74 Surg OncolJournal
Surgical OncologyDOI
10.1016/0960-7404(92)90087-2PubMed ID
1341260Type
ArticleLanguage
enISSN
0960-7404ae974a485f413a2113503eed53cd6c53
10.1016/0960-7404(92)90087-2