Quantitation of enhanced expression of ras-oncogene product (p21) in childhood renal tumours.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108441
Title:
Quantitation of enhanced expression of ras-oncogene product (p21) in childhood renal tumours.
Authors:
Kumar, Shant; Hand, P H; Marsden, Henry B; Kumar, Patricia; Thor, A
Abstract:
A monoclonal antibody, RAP-5, raised against the 21,000d ras-oncogene product, p21, was used in an immunoperoxidase staining procedure to study the expression of p21 in normal children's and foetal kidneys, pre-malignant lesions and benign and malignant childhood renal tumours with good, moderate or poor prognosis. ras-p21 was expressed in both normal and foetal kidneys but its distribution in renal tumours differed markedly (p less than 0.01). A quantitative liquid competition radioimmunoassay (RIA) was used to determine ras-p21 level in tissue homogenates. The results were expressed as pg of ras-p21 per microgram protein of tissue extract. There were significant differences in the levels of ras-p21 among various renal tissue extracts (p less than 0.05). Generally it emerged that the amount of ras-p21 was greater in both malignant renal tumours and foetal kidneys compared to normal kidneys, pre-malignant lesions and benign renal tumours.
Affiliation:
Christie Hospital and Holt Radium Institute, Manchester, England.
Citation:
Quantitation of enhanced expression of ras-oncogene product (p21) in childhood renal tumours., 11 (4):1657-62 Anticancer Res.
Journal:
Anticancer Research
Issue Date:
1991
URI:
http://hdl.handle.net/10541/108441
PubMed ID:
1660693
Type:
Article
Language:
en
ISSN:
0250-7005
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorKumar, Shanten
dc.contributor.authorHand, P Hen
dc.contributor.authorMarsden, Henry Ben
dc.contributor.authorKumar, Patriciaen
dc.contributor.authorThor, Aen
dc.date.accessioned2010-07-27T14:48:52Z-
dc.date.available2010-07-27T14:48:52Z-
dc.date.issued1991-
dc.identifier.citationQuantitation of enhanced expression of ras-oncogene product (p21) in childhood renal tumours., 11 (4):1657-62 Anticancer Res.en
dc.identifier.issn0250-7005-
dc.identifier.pmid1660693-
dc.identifier.urihttp://hdl.handle.net/10541/108441-
dc.description.abstractA monoclonal antibody, RAP-5, raised against the 21,000d ras-oncogene product, p21, was used in an immunoperoxidase staining procedure to study the expression of p21 in normal children's and foetal kidneys, pre-malignant lesions and benign and malignant childhood renal tumours with good, moderate or poor prognosis. ras-p21 was expressed in both normal and foetal kidneys but its distribution in renal tumours differed markedly (p less than 0.01). A quantitative liquid competition radioimmunoassay (RIA) was used to determine ras-p21 level in tissue homogenates. The results were expressed as pg of ras-p21 per microgram protein of tissue extract. There were significant differences in the levels of ras-p21 among various renal tissue extracts (p less than 0.05). Generally it emerged that the amount of ras-p21 was greater in both malignant renal tumours and foetal kidneys compared to normal kidneys, pre-malignant lesions and benign renal tumours.en
dc.language.isoenen
dc.subjectKidney Canceren
dc.subjectBiological Tumour Markersen
dc.subjectWilms Tumouren
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshCarcinoma, Renal Cell-
dc.subject.meshChild-
dc.subject.meshFetus-
dc.subject.meshGenes, ras-
dc.subject.meshHumans-
dc.subject.meshImmunoenzyme Techniques-
dc.subject.meshKidney-
dc.subject.meshKidney Neoplasms-
dc.subject.meshPrecancerous Conditions-
dc.subject.meshProto-Oncogene Proteins p21(ras)-
dc.subject.meshRadioimmunoassay-
dc.subject.meshReference Values-
dc.subject.meshTumor Markers, Biological-
dc.subject.meshWilms Tumor-
dc.titleQuantitation of enhanced expression of ras-oncogene product (p21) in childhood renal tumours.en
dc.typeArticleen
dc.contributor.departmentChristie Hospital and Holt Radium Institute, Manchester, England.en
dc.identifier.journalAnticancer Researchen

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