Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calcium.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108403
Title:
Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calcium.
Authors:
Talbot, J A; Lambert, A; Mitchell, R; Grabinski, M; Anderson, D C; Tsatsoulis, Agathocles; Shalet, Stephen M; Robertson, W R
Abstract:
We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/l) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091 +/- 322 to 1480 +/- 84 pmol/l (p less than 0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360 +/- 45 to 1242 +/- 133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 mumol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 mumol/l) and A23187 (2 mumol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by greater than 80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 mumol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 mumol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.
Affiliation:
Department of Medicine (Clinical Biochemistry), University of Manchester, Hope Hospital, Salford, UK.
Citation:
Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calcium. 1991, 125 (3):280-5 Acta Endocrinol.
Journal:
Acta Endocrinologica
Issue Date:
Sep-1991
URI:
http://hdl.handle.net/10541/108403
DOI:
10.1530/acta.0.1250280
PubMed ID:
1659097
Type:
Article
Language:
en
ISSN:
0001-5598
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorTalbot, J Aen
dc.contributor.authorLambert, Aen
dc.contributor.authorMitchell, Ren
dc.contributor.authorGrabinski, Men
dc.contributor.authorAnderson, D Cen
dc.contributor.authorTsatsoulis, Agathoclesen
dc.contributor.authorShalet, Stephen Men
dc.contributor.authorRobertson, W Ren
dc.date.accessioned2010-07-27T10:52:04Z-
dc.date.available2010-07-27T10:52:04Z-
dc.date.issued1991-09-
dc.identifier.citationFollicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calcium. 1991, 125 (3):280-5 Acta Endocrinol.en
dc.identifier.issn0001-5598-
dc.identifier.pmid1659097-
dc.identifier.doi10.1530/acta.0.1250280-
dc.identifier.urihttp://hdl.handle.net/10541/108403-
dc.description.abstractWe have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/l) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091 +/- 322 to 1480 +/- 84 pmol/l (p less than 0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360 +/- 45 to 1242 +/- 133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 mumol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 mumol/l) and A23187 (2 mumol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by greater than 80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 mumol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 mumol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.en
dc.language.isoenen
dc.subjectOestrogensen
dc.subject.meshAnimals-
dc.subject.meshBucladesine-
dc.subject.meshCalcimycin-
dc.subject.meshCalcium-
dc.subject.meshCells, Cultured-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshDrug Interactions-
dc.subject.meshEgtazic Acid-
dc.subject.meshEstradiol-
dc.subject.meshEstrogens-
dc.subject.meshFollicle Stimulating Hormone-
dc.subject.meshForskolin-
dc.subject.meshIonomycin-
dc.subject.meshMale-
dc.subject.meshRadioimmunoassay-
dc.subject.meshRats-
dc.subject.meshRats, Inbred Strains-
dc.subject.meshSertoli Cells-
dc.titleFollicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calcium.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medicine (Clinical Biochemistry), University of Manchester, Hope Hospital, Salford, UK.en
dc.identifier.journalActa Endocrinologicaen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.