2.50
Hdl Handle:
http://hdl.handle.net/10541/108142
Title:
Haemopoietic cell kinetics in humans treated with rGM-CSF.
Authors:
Lord, Brian I; Gurney, H; Chang, James; Thatcher, Nick; Crowther, Derek; Dexter, T Michael
Abstract:
We have investigated the kinetics of myeloid cell proliferation in the marrow of patients with small-cell lung cancer and treated with 10 daily subcutaneous injections of granulocyte/macrophage colony-stimulating factor (GM-CSF). Bone marrow, obtained before and during treatment with the growth factor, was labelled in vitro with tritiated thymidine (3H-TdR). A 3rd bone-marrow sample was obtained 1 hr following an intravenous injection of 3H-TdR. Subsequent daily blood samples were also collected, and 3H-TdR labelling was assessed on these and the marrow preparations by autoradiography. GM-CSF treatment increased the peripheral granulocytic cells nearly 5-fold, but this included significant eosinophilia, so that the neutrophilic granulocytes increased only 3.3-fold. These cells were released from the marrow over a normal time scale, but their peripheral half-life was about 6 times longer than normal and they were probably functionally defective. Furthermore, significant numbers of immature cells were released from the marrow. Neutrophil production stimulated by GM-CSF was thus overestimated by measurement of the apparent peripheral granulocytosis. Increased labelling indices and grain counts in the proliferating granulocytic cells of the marrow indicate shortened cell-cycle times, and the excess granulocyte production appears to be the result of extra amplification divisions in the proliferative compartments.
Affiliation:
Cancer Research Campaign Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, UK.
Citation:
Haemopoietic cell kinetics in humans treated with rGM-CSF. 1992, 50 (1):26-31 Int J Cancer
Journal:
International Journal of Cancer
Issue Date:
2-Jan-1992
URI:
http://hdl.handle.net/10541/108142
PubMed ID:
1370226
Type:
Article
Language:
en
ISSN:
0020-7136
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLord, Brian Ien
dc.contributor.authorGurney, Hen
dc.contributor.authorChang, Jamesen
dc.contributor.authorThatcher, Nicken
dc.contributor.authorCrowther, Dereken
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-07-22T09:08:01Z-
dc.date.available2010-07-22T09:08:01Z-
dc.date.issued1992-01-02-
dc.identifier.citationHaemopoietic cell kinetics in humans treated with rGM-CSF. 1992, 50 (1):26-31 Int J Canceren
dc.identifier.issn0020-7136-
dc.identifier.pmid1370226-
dc.identifier.urihttp://hdl.handle.net/10541/108142-
dc.description.abstractWe have investigated the kinetics of myeloid cell proliferation in the marrow of patients with small-cell lung cancer and treated with 10 daily subcutaneous injections of granulocyte/macrophage colony-stimulating factor (GM-CSF). Bone marrow, obtained before and during treatment with the growth factor, was labelled in vitro with tritiated thymidine (3H-TdR). A 3rd bone-marrow sample was obtained 1 hr following an intravenous injection of 3H-TdR. Subsequent daily blood samples were also collected, and 3H-TdR labelling was assessed on these and the marrow preparations by autoradiography. GM-CSF treatment increased the peripheral granulocytic cells nearly 5-fold, but this included significant eosinophilia, so that the neutrophilic granulocytes increased only 3.3-fold. These cells were released from the marrow over a normal time scale, but their peripheral half-life was about 6 times longer than normal and they were probably functionally defective. Furthermore, significant numbers of immature cells were released from the marrow. Neutrophil production stimulated by GM-CSF was thus overestimated by measurement of the apparent peripheral granulocytosis. Increased labelling indices and grain counts in the proliferating granulocytic cells of the marrow indicate shortened cell-cycle times, and the excess granulocyte production appears to be the result of extra amplification divisions in the proliferative compartments.en
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshCarcinoma, Small Cell-
dc.subject.meshCell Cycle-
dc.subject.meshGranulocyte Colony-Stimulating Factor-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshGranulocytes-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshLeukocyte Count-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMonocytes-
dc.subject.meshRecombinant Proteins-
dc.titleHaemopoietic cell kinetics in humans treated with rGM-CSF.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalInternational Journal of Canceren

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