In vitro and in vivo analysis of the effects of recombinant human granulocyte colony-stimulating factor in patients.
Authors
Bronchud, M HPotter, M R
Morgenstern, Godfrey R
Blasco, M J
Scarffe, J Howard
Thatcher, Nick
Crowther, Derek
Souza, L M
Alton, N K
Testa, Nydia G
Dexter, T Michael
Affiliation
Department of Medical Oncology, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.Issue Date
1988-07
Metadata
Show full item recordAbstract
Twelve patients with small cell lung cancer were treated with recombinant human granulocyte colony-stimulating factor, rhG-CSF, given by continuous infusion at doses ranging from 1 to 40 micrograms kg-1 day-1. Patients received the rhG-CSF before the start of intensive chemotherapy and after alternate cycles of chemotherapy. Several in vitro assays were performed using peripheral blood neutrophils and marrow progenitor cells collected from patients prior to and after infusion of the growth factor. Peripheral blood neutrophils were tested for mobility and phagocytic activity. In addition, in vitro clonogenic assays of marrow haemopoietic progenitor cells and analysis of bone marrow trephines and aspirates were carried out. We found that rhG-CSF in vivo has at least two main effects: (a) an early fall in peripheral neutrophils, within the first hour, followed by a rapid influx of mature neutrophils into the circulatory pool; (b) stimulation of proliferation and differentiation of neutrophil precursors in the bone marrow. Neutrophils released into the circulation were normal in tests of their mobility and phagocytic activity.Citation
In vitro and in vivo analysis of the effects of recombinant human granulocyte colony-stimulating factor in patients. 1988, 58 (1):64-9 Br. J. CancerJournal
British Journal of CancerPubMed ID
2458748Type
ArticleLanguage
enISSN
0007-0920Related articles
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