A simple quantitative HPLC assay for ifosfamide in biological fluids.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108030
Title:
A simple quantitative HPLC assay for ifosfamide in biological fluids.
Authors:
Margison, Jennifer M; Wilkinson, Peter M; Cerny, T; Thatcher, Nick
Abstract:
A high performance liquid chromatography method is described for measuring Ifosfamide (I) in human serum. This involves solvent extraction, reverse phase HPLC and UV detection at 190 nm. Standard curves of peak height x detector sensitivity versus I concentration in serum were linear with a lower limit of detection of 100 ng/ml. Authentic 14C-labelled I cochromatographed with standard I and with I found in serum from treated patients. The concentration-time curves of I determined by both HPLC and gas chromatography were indistinguishable. We conclude that this method is suitable for determining I pharmacokinetics in biological specimens.
Affiliation:
Department of Clinical Pharmacology, Christie Hospital, Holt Radium Institute, Manchester, UK.
Citation:
A simple quantitative HPLC assay for ifosfamide in biological fluids. 1986, 1 (3):101-3 Biomed. Chromatogr.
Journal:
Biomedical Chromatography
Issue Date:
Jun-1986
URI:
http://hdl.handle.net/10541/108030
DOI:
10.1002/bmc.1130010303
PubMed ID:
3506819
Type:
Article
Language:
en
ISSN:
0269-3879
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorMargison, Jennifer Men
dc.contributor.authorWilkinson, Peter Men
dc.contributor.authorCerny, Ten
dc.contributor.authorThatcher, Nicken
dc.date.accessioned2010-07-21T10:15:23Z-
dc.date.available2010-07-21T10:15:23Z-
dc.date.issued1986-06-
dc.identifier.citationA simple quantitative HPLC assay for ifosfamide in biological fluids. 1986, 1 (3):101-3 Biomed. Chromatogr.en
dc.identifier.issn0269-3879-
dc.identifier.pmid3506819-
dc.identifier.doi10.1002/bmc.1130010303-
dc.identifier.urihttp://hdl.handle.net/10541/108030-
dc.description.abstractA high performance liquid chromatography method is described for measuring Ifosfamide (I) in human serum. This involves solvent extraction, reverse phase HPLC and UV detection at 190 nm. Standard curves of peak height x detector sensitivity versus I concentration in serum were linear with a lower limit of detection of 100 ng/ml. Authentic 14C-labelled I cochromatographed with standard I and with I found in serum from treated patients. The concentration-time curves of I determined by both HPLC and gas chromatography were indistinguishable. We conclude that this method is suitable for determining I pharmacokinetics in biological specimens.en
dc.language.isoenen
dc.subject.meshCarbon Radioisotopes-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshHumans-
dc.subject.meshIfosfamide-
dc.subject.meshSpectrophotometry, Ultraviolet-
dc.titleA simple quantitative HPLC assay for ifosfamide in biological fluids.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Pharmacology, Christie Hospital, Holt Radium Institute, Manchester, UK.en
dc.identifier.journalBiomedical Chromatographyen
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