A low-temperature method for the isolation of small-intestinal epithelium along the crypt-villus axis.

2.50
Hdl Handle:
http://hdl.handle.net/10541/106582
Title:
A low-temperature method for the isolation of small-intestinal epithelium along the crypt-villus axis.
Authors:
Flint, Neil; Cove, Frances L; Evans, Gareth S
Abstract:
A variety of enzymic and non-enzymic methods to isolate epithelium from the small intestine have been previously published. Sequential fractionation of cells from the villus to the crypt has been reported in some of these papers, which allows the comparative study of terminally differentiated and proliferative cell phenotypes. However, these methods often involve the incubation of tissues at 37 degrees C, which may affect the structural and biochemical integrity of the cells. We have developed a rapid low-temperature (4 degrees C) method for isolating purified populations of crypt and villus cells from mouse and rat intestines. The fractionated cells have been partially characterized, and the potential value of the procedure has been indicated by the ability to analyse the comparative protein and mRNA expression along the crypt-villus axis.
Affiliation:
Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital, Withington, Manchester, U.K.
Citation:
A low-temperature method for the isolation of small-intestinal epithelium along the crypt-villus axis. 1991, 280 ( Pt 2):331-4 Biochem. J.
Journal:
Biochemical Journal
Issue Date:
1-Dec-1991
URI:
http://hdl.handle.net/10541/106582
PubMed ID:
1747105
Type:
Article
Language:
en
ISSN:
0264-6021
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorFlint, Neilen
dc.contributor.authorCove, Frances Len
dc.contributor.authorEvans, Gareth Sen
dc.date.accessioned2010-06-21T14:04:49Z-
dc.date.available2010-06-21T14:04:49Z-
dc.date.issued1991-12-01-
dc.identifier.citationA low-temperature method for the isolation of small-intestinal epithelium along the crypt-villus axis. 1991, 280 ( Pt 2):331-4 Biochem. J.en
dc.identifier.issn0264-6021-
dc.identifier.pmid1747105-
dc.identifier.urihttp://hdl.handle.net/10541/106582-
dc.description.abstractA variety of enzymic and non-enzymic methods to isolate epithelium from the small intestine have been previously published. Sequential fractionation of cells from the villus to the crypt has been reported in some of these papers, which allows the comparative study of terminally differentiated and proliferative cell phenotypes. However, these methods often involve the incubation of tissues at 37 degrees C, which may affect the structural and biochemical integrity of the cells. We have developed a rapid low-temperature (4 degrees C) method for isolating purified populations of crypt and villus cells from mouse and rat intestines. The fractionated cells have been partially characterized, and the potential value of the procedure has been indicated by the ability to analyse the comparative protein and mRNA expression along the crypt-villus axis.en
dc.language.isoenen
dc.subject.meshAlkaline Phosphatase-
dc.subject.meshAnimals-
dc.subject.meshCell Fractionation-
dc.subject.meshCells, Cultured-
dc.subject.meshCold Temperature-
dc.subject.meshElectrophoresis, Agar Gel-
dc.subject.meshEpithelial Cells-
dc.subject.meshEpithelium-
dc.subject.meshIntestine, Small-
dc.subject.meshMale-
dc.subject.meshProteins-
dc.subject.meshRNA, Messenger-
dc.subject.meshRats-
dc.subject.meshRats, Inbred Strains-
dc.subject.meshThymidine-
dc.titleA low-temperature method for the isolation of small-intestinal epithelium along the crypt-villus axis.en
dc.typeArticleen
dc.contributor.departmentDepartment of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital, Withington, Manchester, U.K.en
dc.identifier.journalBiochemical Journalen
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