Immunoglobulin heavy chain gene fingerprinting reveals widespread oligoclonality in B-lineage acute lymphoblastic leukaemia.

2.50
Hdl Handle:
http://hdl.handle.net/10541/106577
Title:
Immunoglobulin heavy chain gene fingerprinting reveals widespread oligoclonality in B-lineage acute lymphoblastic leukaemia.
Authors:
Deane, M; Pappas, H; Norton, John D
Abstract:
Acute lymphoblastic leukaemia (ALL) of B-cell lineage typically arises as a monoclonal expansion of committed B-lymphocyte precursors that are arrested at an immature stage of differentiation. From Southern hybridization analysis of immunoglobulin heavy chain (IgH) genes in leukaemic blasts, the occurrence of a sizeable minority of patients displaying multiple (greater than two) rearranged heavy chain alleles has been widely reported. In at least some patients these data are consistent with the presence of oligoclonal populations of precursor B-cells. We have used a more sensitive, polymerase chain reaction based immunoglobulin gene 'fingerprinting' approach in the analysis of B-cell clonality in eight patients with common ALL which were apparently monoclonal on the basis of Southern blot analysis of their IgH genes. The results revealed an oligoclonal pattern of IgH gene rearrangement in half of the patients analysed, implying that oligoclonality at the level of B-cell commitment, as defined by IgH gene rearrangement, is much more widespread in this disease than has previously been recognized.
Affiliation:
Department of Haematology, Royal Free Hospital School of Medicine, London, UK.
Citation:
Immunoglobulin heavy chain gene fingerprinting reveals widespread oligoclonality in B-lineage acute lymphoblastic leukaemia. 1991, 5 (10):832-8 Leukemia
Journal:
Leukemia
Issue Date:
Oct-1991
URI:
http://hdl.handle.net/10541/106577
PubMed ID:
1961017
Type:
Article
Language:
en
ISSN:
0887-6924
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorDeane, Men
dc.contributor.authorPappas, Hen
dc.contributor.authorNorton, John Den
dc.date.accessioned2010-06-21T14:00:17Z-
dc.date.available2010-06-21T14:00:17Z-
dc.date.issued1991-10-
dc.identifier.citationImmunoglobulin heavy chain gene fingerprinting reveals widespread oligoclonality in B-lineage acute lymphoblastic leukaemia. 1991, 5 (10):832-8 Leukemiaen
dc.identifier.issn0887-6924-
dc.identifier.pmid1961017-
dc.identifier.urihttp://hdl.handle.net/10541/106577-
dc.description.abstractAcute lymphoblastic leukaemia (ALL) of B-cell lineage typically arises as a monoclonal expansion of committed B-lymphocyte precursors that are arrested at an immature stage of differentiation. From Southern hybridization analysis of immunoglobulin heavy chain (IgH) genes in leukaemic blasts, the occurrence of a sizeable minority of patients displaying multiple (greater than two) rearranged heavy chain alleles has been widely reported. In at least some patients these data are consistent with the presence of oligoclonal populations of precursor B-cells. We have used a more sensitive, polymerase chain reaction based immunoglobulin gene 'fingerprinting' approach in the analysis of B-cell clonality in eight patients with common ALL which were apparently monoclonal on the basis of Southern blot analysis of their IgH genes. The results revealed an oligoclonal pattern of IgH gene rearrangement in half of the patients analysed, implying that oligoclonality at the level of B-cell commitment, as defined by IgH gene rearrangement, is much more widespread in this disease than has previously been recognized.en
dc.language.isoenen
dc.subjectCancer DNAen
dc.subjectPrecursor Cell Lymphoblastic Leukaemia-Lymphomaen
dc.subject.meshAlleles-
dc.subject.meshBlotting, Southern-
dc.subject.meshBurkitt Lymphoma-
dc.subject.meshClone Cells-
dc.subject.meshDNA Fingerprinting-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshGene Rearrangement, B-Lymphocyte, Heavy Chain-
dc.subject.meshHumans-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshPrecursor Cell Lymphoblastic Leukemia-Lymphoma-
dc.titleImmunoglobulin heavy chain gene fingerprinting reveals widespread oligoclonality in B-lineage acute lymphoblastic leukaemia.en
dc.typeArticleen
dc.contributor.departmentDepartment of Haematology, Royal Free Hospital School of Medicine, London, UK.en
dc.identifier.journalLeukemiaen

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