Monoclonal antibodies to human embryonal carcinoma cells: antigenic relationships of germ cell tumors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/106576
Title:
Monoclonal antibodies to human embryonal carcinoma cells: antigenic relationships of germ cell tumors.
Authors:
Rinke de Wit, T F; Wilson, L; Van den Elsen, P J; Thielen, F; Brekhoff, D; Oosterhuis, J W; Pera, M F; Stern, Peter L
Abstract:
Fifteen monoclonal antibodies (mAb) that show specificity for human embryonal carcinoma cells are described. C57BL/6 mice were immunized with Tera-2 embryonal carcinoma cells, and hybridomas were isolated and tested versus a set of human developmental tumor cell lines. The antigens exhibit relatively restricted and unique distributions on normal tissues as shown by immunohistochemistry. The mAbs recognize a series of differentiation antigens since their expression changes when teratocarcinoma stem cells are induced to differentiate in vitro. The expression of these molecules in germ cell and related tumors is consistent with the data obtained from in vitro cell studies. Seven of these mAbs immunoprecipitate molecules from surface labeled Tera-2 cells that show distinct molecular weights on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antigenic phenotypes defined by the set of mAbs can be used to investigate possible pathogenetic relationships of the various testicular tumors.
Affiliation:
Department of Immunohematology, Leiden University Hospital, REP-TNO, Rijswijk, The Netherlands.
Citation:
Monoclonal antibodies to human embryonal carcinoma cells: antigenic relationships of germ cell tumors. 1991, 65 (2):180-91 Lab. Invest.
Journal:
Laboratory Investigation
Issue Date:
Aug-1991
URI:
http://hdl.handle.net/10541/106576
PubMed ID:
1881121
Type:
Article
Language:
en
ISSN:
0023-6837
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorRinke de Wit, T Fen
dc.contributor.authorWilson, Len
dc.contributor.authorVan den Elsen, P Jen
dc.contributor.authorThielen, Fen
dc.contributor.authorBrekhoff, Den
dc.contributor.authorOosterhuis, J Wen
dc.contributor.authorPera, M Fen
dc.contributor.authorStern, Peter Len
dc.date.accessioned2010-06-21T13:58:26Z-
dc.date.available2010-06-21T13:58:26Z-
dc.date.issued1991-08-
dc.identifier.citationMonoclonal antibodies to human embryonal carcinoma cells: antigenic relationships of germ cell tumors. 1991, 65 (2):180-91 Lab. Invest.en
dc.identifier.issn0023-6837-
dc.identifier.pmid1881121-
dc.identifier.urihttp://hdl.handle.net/10541/106576-
dc.description.abstractFifteen monoclonal antibodies (mAb) that show specificity for human embryonal carcinoma cells are described. C57BL/6 mice were immunized with Tera-2 embryonal carcinoma cells, and hybridomas were isolated and tested versus a set of human developmental tumor cell lines. The antigens exhibit relatively restricted and unique distributions on normal tissues as shown by immunohistochemistry. The mAbs recognize a series of differentiation antigens since their expression changes when teratocarcinoma stem cells are induced to differentiate in vitro. The expression of these molecules in germ cell and related tumors is consistent with the data obtained from in vitro cell studies. Seven of these mAbs immunoprecipitate molecules from surface labeled Tera-2 cells that show distinct molecular weights on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antigenic phenotypes defined by the set of mAbs can be used to investigate possible pathogenetic relationships of the various testicular tumors.en
dc.language.isoenen
dc.subjectCancer Antigensen
dc.subjectCancerous Stem Cellsen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAnimals-
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshAntibody Specificity-
dc.subject.meshAntigens, Differentiation-
dc.subject.meshAntigens, Neoplasm-
dc.subject.meshEmbryonal Carcinoma Stem Cells-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshNeoplastic Stem Cells-
dc.subject.meshPrecipitin Tests-
dc.subject.meshTumor Cells, Cultured-
dc.titleMonoclonal antibodies to human embryonal carcinoma cells: antigenic relationships of germ cell tumors.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunohematology, Leiden University Hospital, REP-TNO, Rijswijk, The Netherlands.en
dc.identifier.journalLaboratory Investigationen
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