Acute response of mouse kidney clonogens to fractionated irradiation in situ and then assayed in primary culture.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104748
Title:
Acute response of mouse kidney clonogens to fractionated irradiation in situ and then assayed in primary culture.
Authors:
Jen, Yee-Min; Hendry, Jolyon H
Abstract:
Although the difference in sensitivity to the changes in dose fraction size between early-responding and late-responding tissues is well established, the underlying mechanisms in terms of target-cell responses are not yet clearly identified for any tissue. The radiosensitivity of mouse kidney cells after in situ single-dose, 2, 8, and 16 fraction X-irradiations was measured in primary culture using a clonogenic assay. The assay was made 12 h after single doses or 12 h after the last dose of the multifraction regimens. When analysed using the linear-quadratic model, as predicted the individual alpha components for all the different fractionation schedules were not significantly different, and the changes in the beta values were consistent with those expected on the basis of the reciprocal fraction numbers. When all four data sets were integrated to derive a common alpha/beta ratio, the result was 4.4 +/- 1.3 (1SE) Gy, or 2.8 +/- 0.9 Gy (a better fit) if the single-dose data set was excluded. These values fall into the range reported for kidney using assays of tissue function at long times after irradiation. Hence, it has been shown for the first time that the fractionation sensitivity of a late-responding organ is mimicked by that of a clonogenic cell population in that organ. The evidence also suggests that the time available prior to fixation of potentially lethal damage does not influence the low alpha/beta ratio observed for the kidney.
Affiliation:
Cancer Research Campaign Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
Citation:
Acute response of mouse kidney clonogens to fractionated irradiation in situ and then assayed in primary culture. 1991, 64 (758):142-8 Br J Radiol
Journal:
British Journal of Radiology
Issue Date:
Feb-1991
URI:
http://hdl.handle.net/10541/104748
DOI:
10.1259/0007-1285-64-758-142
PubMed ID:
2004205
Type:
Article
Language:
en
ISSN:
0007-1285
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorJen, Yee-Minen
dc.contributor.authorHendry, Jolyon Hen
dc.date.accessioned2010-06-11T15:52:31Z-
dc.date.available2010-06-11T15:52:31Z-
dc.date.issued1991-02-
dc.identifier.citationAcute response of mouse kidney clonogens to fractionated irradiation in situ and then assayed in primary culture. 1991, 64 (758):142-8 Br J Radiolen
dc.identifier.issn0007-1285-
dc.identifier.pmid2004205-
dc.identifier.doi10.1259/0007-1285-64-758-142-
dc.identifier.urihttp://hdl.handle.net/10541/104748-
dc.description.abstractAlthough the difference in sensitivity to the changes in dose fraction size between early-responding and late-responding tissues is well established, the underlying mechanisms in terms of target-cell responses are not yet clearly identified for any tissue. The radiosensitivity of mouse kidney cells after in situ single-dose, 2, 8, and 16 fraction X-irradiations was measured in primary culture using a clonogenic assay. The assay was made 12 h after single doses or 12 h after the last dose of the multifraction regimens. When analysed using the linear-quadratic model, as predicted the individual alpha components for all the different fractionation schedules were not significantly different, and the changes in the beta values were consistent with those expected on the basis of the reciprocal fraction numbers. When all four data sets were integrated to derive a common alpha/beta ratio, the result was 4.4 +/- 1.3 (1SE) Gy, or 2.8 +/- 0.9 Gy (a better fit) if the single-dose data set was excluded. These values fall into the range reported for kidney using assays of tissue function at long times after irradiation. Hence, it has been shown for the first time that the fractionation sensitivity of a late-responding organ is mimicked by that of a clonogenic cell population in that organ. The evidence also suggests that the time available prior to fixation of potentially lethal damage does not influence the low alpha/beta ratio observed for the kidney.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Survival-
dc.subject.meshCells, Cultured-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshKidney-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshTime Factors-
dc.titleAcute response of mouse kidney clonogens to fractionated irradiation in situ and then assayed in primary culture.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.en
dc.identifier.journalBritish Journal of Radiologyen
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