High-level expression of the Epstein-Barr virus alkaline deoxyribonuclease using a recombinant baculovirus: application to the diagnosis of nasopharyngeal carcinoma.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104745
Title:
High-level expression of the Epstein-Barr virus alkaline deoxyribonuclease using a recombinant baculovirus: application to the diagnosis of nasopharyngeal carcinoma.
Authors:
Baylis, Sally A; Purifoy, D J; Littler, Edward
Abstract:
The Epstein-Barr virus (EBV) alkaline deoxyribonuclease (DNase) was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV). Infection of the insect cell line Spodoptera frugiperda (SF9) with the recombinant virus led to the expression of an enzymatically active alkaline DNase. The recombinant EBV alkaline DNase was highly soluble, and the recombinant baculovirus produced approximately 10-20 mg of EBV DNase per 1 X 10(9) cells. The recombinant enzyme activity was neutralized by specific antisera to the EBV DNase and was recognized by these sera in Western blot analysis and immunofluorescence tests. The recombinant EBV DNase was neutralized by these sera from patients with nasopharyngeal carcinoma and chronic infectious mononucleosis. Western blot analysis using these patients' sera showed that IgG and IgA antibodies to the EBV DNase could be readily detected.
Affiliation:
Cancer Research Campaign Laboratories, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Withington, Manchester, United Kingdom.
Citation:
High-level expression of the Epstein-Barr virus alkaline deoxyribonuclease using a recombinant baculovirus: application to the diagnosis of nasopharyngeal carcinoma. 1991, 181 (1):390-4 Virology
Journal:
Virology
Issue Date:
Mar-1991
URI:
http://hdl.handle.net/10541/104745
DOI:
10.1016/0042-6822(91)90511-9
PubMed ID:
1847261
Type:
Article
Language:
en
ISSN:
0042-6822
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBaylis, Sally Aen
dc.contributor.authorPurifoy, D Jen
dc.contributor.authorLittler, Edwarden
dc.date.accessioned2010-06-11T15:38:08Z-
dc.date.available2010-06-11T15:38:08Z-
dc.date.issued1991-03-
dc.identifier.citationHigh-level expression of the Epstein-Barr virus alkaline deoxyribonuclease using a recombinant baculovirus: application to the diagnosis of nasopharyngeal carcinoma. 1991, 181 (1):390-4 Virologyen
dc.identifier.issn0042-6822-
dc.identifier.pmid1847261-
dc.identifier.doi10.1016/0042-6822(91)90511-9-
dc.identifier.urihttp://hdl.handle.net/10541/104745-
dc.description.abstractThe Epstein-Barr virus (EBV) alkaline deoxyribonuclease (DNase) was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV). Infection of the insect cell line Spodoptera frugiperda (SF9) with the recombinant virus led to the expression of an enzymatically active alkaline DNase. The recombinant EBV alkaline DNase was highly soluble, and the recombinant baculovirus produced approximately 10-20 mg of EBV DNase per 1 X 10(9) cells. The recombinant enzyme activity was neutralized by specific antisera to the EBV DNase and was recognized by these sera in Western blot analysis and immunofluorescence tests. The recombinant EBV DNase was neutralized by these sera from patients with nasopharyngeal carcinoma and chronic infectious mononucleosis. Western blot analysis using these patients' sera showed that IgG and IgA antibodies to the EBV DNase could be readily detected.en
dc.language.isoenen
dc.subjectNasopharyngeal Canceren
dc.subject.meshAnimals-
dc.subject.meshBaculoviridae-
dc.subject.meshBlotting, Western-
dc.subject.meshCell Line-
dc.subject.meshDeoxyribonucleases-
dc.subject.meshElectrophoresis, Polyacrylamide Gel-
dc.subject.meshFluorescent Antibody Technique-
dc.subject.meshHerpesvirus 4, Human-
dc.subject.meshHumans-
dc.subject.meshKinetics-
dc.subject.meshMolecular Weight-
dc.subject.meshNasopharyngeal Neoplasms-
dc.subject.meshPlasmids-
dc.subject.meshTransfection-
dc.titleHigh-level expression of the Epstein-Barr virus alkaline deoxyribonuclease using a recombinant baculovirus: application to the diagnosis of nasopharyngeal carcinoma.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Laboratories, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Withington, Manchester, United Kingdom.en
dc.identifier.journalVirologyen

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