Effects of the steel gene product on mouse primordial germ cells in culture.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104742
Title:
Effects of the steel gene product on mouse primordial germ cells in culture.
Authors:
Godin, I; Deed, Richard W; Cooke, J; Zsebo, K; Dexter, T Michael; Wylie, C C
Abstract:
Mutations at the steel (sl) and dominant white spotting (W) loci in the mouse affect primordial germ cells (PGC), melanoblasts and haemopoietic stem cells. The W gene encodes a cell-surface receptor of the tyrosine kinase family, the proto-oncogene c-kit. In situ analysis has shown c-kit messenger RNA expression in PGC in the early genital ridges. The Sl gene encodes the ligand for this receptor, a peptide growth factor, called here stem cell factor (SCF). SCF mRNA is expressed in many regions of the early mouse embryo, including the areas of migration of these cell types. It is important now to identify the role of the Sl-W interaction in the development of these migratory embryonic stem cell populations. Using an in vitro assay system, we show that SCF increases both the overall numbers and colony sizes of migratory PGC isolated from wild-type mouse embryos, and cultured on irradiated feeder layers of STO cells (a mouse embryonic fibroblast line). In the absence of feeder cells, SCF causes a large increase in the initial survival and apparent motility of PGC in culture. But labelling with bromodeoxyuridine shows that SCF is not, by itself, a mitogen for PGC. SCF does not exert a chemotropic effect on PGC in in vitro assays. These results suggest that SCF in vivo is an essential requirement for PGC survival. This demonstrates the control of the early germ-line population by a specific trophic factor.
Affiliation:
Wellcome/CRC Institute, University of Cambridge, UK.
Citation:
Effects of the steel gene product on mouse primordial germ cells in culture. 1991, 352 (6338):807-9 Nature
Journal:
Nature
Issue Date:
29-Aug-1991
URI:
http://hdl.handle.net/10541/104742
DOI:
10.1038/352807a0
PubMed ID:
1715517
Type:
Article
Language:
en
ISSN:
0028-0836
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGodin, Ien
dc.contributor.authorDeed, Richard Wen
dc.contributor.authorCooke, Jen
dc.contributor.authorZsebo, Ken
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorWylie, C Cen
dc.date.accessioned2010-06-11T15:18:32Z-
dc.date.available2010-06-11T15:18:32Z-
dc.date.issued1991-08-29-
dc.identifier.citationEffects of the steel gene product on mouse primordial germ cells in culture. 1991, 352 (6338):807-9 Natureen
dc.identifier.issn0028-0836-
dc.identifier.pmid1715517-
dc.identifier.doi10.1038/352807a0-
dc.identifier.urihttp://hdl.handle.net/10541/104742-
dc.description.abstractMutations at the steel (sl) and dominant white spotting (W) loci in the mouse affect primordial germ cells (PGC), melanoblasts and haemopoietic stem cells. The W gene encodes a cell-surface receptor of the tyrosine kinase family, the proto-oncogene c-kit. In situ analysis has shown c-kit messenger RNA expression in PGC in the early genital ridges. The Sl gene encodes the ligand for this receptor, a peptide growth factor, called here stem cell factor (SCF). SCF mRNA is expressed in many regions of the early mouse embryo, including the areas of migration of these cell types. It is important now to identify the role of the Sl-W interaction in the development of these migratory embryonic stem cell populations. Using an in vitro assay system, we show that SCF increases both the overall numbers and colony sizes of migratory PGC isolated from wild-type mouse embryos, and cultured on irradiated feeder layers of STO cells (a mouse embryonic fibroblast line). In the absence of feeder cells, SCF causes a large increase in the initial survival and apparent motility of PGC in culture. But labelling with bromodeoxyuridine shows that SCF is not, by itself, a mitogen for PGC. SCF does not exert a chemotropic effect on PGC in in vitro assays. These results suggest that SCF in vivo is an essential requirement for PGC survival. This demonstrates the control of the early germ-line population by a specific trophic factor.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBromodeoxyuridine-
dc.subject.meshCell Count-
dc.subject.meshCell Division-
dc.subject.meshCell Movement-
dc.subject.meshCell Survival-
dc.subject.meshCells, Cultured-
dc.subject.meshFibroblasts-
dc.subject.meshGerm Cells-
dc.subject.meshHematopoietic Cell Growth Factors-
dc.subject.meshMice-
dc.subject.meshProto-Oncogene Proteins-
dc.subject.meshProto-Oncogene Proteins c-kit-
dc.subject.meshRNA, Messenger-
dc.subject.meshStem Cell Factor-
dc.titleEffects of the steel gene product on mouse primordial germ cells in culture.en
dc.typeArticleen
dc.contributor.departmentWellcome/CRC Institute, University of Cambridge, UK.en
dc.identifier.journalNatureen

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