2.50
Hdl Handle:
http://hdl.handle.net/10541/104735
Title:
Clonogen number and radiosensitivity in rat thyroid follicles.
Authors:
Watanabe, H; Hendry, Jolyon H
Abstract:
A technique has been developed for transplanting whole thyroid follicles into the fat pads of recipient thyroidectomized rats to assess the ability of the follicular cells to proliferate and form colonies, i.e., clusters of new follicles. Of the transplanted follicles, 80-90% formed follicle clusters, indicative of the presence of transplanted follicles not containing colony-forming cells (clonogens) or some reproducible degree of transplantation trauma. The initial number of clonogens per regenerative follicle was calculated from data from split-dose experiments to be 3.3 +/- 1.5, and their sensitivity was characterized by a D0 value of 350 +/- 95 cGy. Three clonogens among about 80 epithelial cells per regenerative follicle and the 10-20% of nonregenerative follicles represent an overall colony-forming efficiency of about 3%. This is similar to the value of 2-3% reported by others using single-cell transplantation techniques.
Affiliation:
Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.
Citation:
Clonogen number and radiosensitivity in rat thyroid follicles. 1991, 128 (2):222-4 Radiat. Res.
Journal:
Radiation Research
Issue Date:
Nov-1991
URI:
http://hdl.handle.net/10541/104735
PubMed ID:
1947019
Type:
Article
Language:
en
ISSN:
0033-7587
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWatanabe, Hen
dc.contributor.authorHendry, Jolyon Hen
dc.date.accessioned2010-06-11T14:52:44Z-
dc.date.available2010-06-11T14:52:44Z-
dc.date.issued1991-11-
dc.identifier.citationClonogen number and radiosensitivity in rat thyroid follicles. 1991, 128 (2):222-4 Radiat. Res.en
dc.identifier.issn0033-7587-
dc.identifier.pmid1947019-
dc.identifier.urihttp://hdl.handle.net/10541/104735-
dc.description.abstractA technique has been developed for transplanting whole thyroid follicles into the fat pads of recipient thyroidectomized rats to assess the ability of the follicular cells to proliferate and form colonies, i.e., clusters of new follicles. Of the transplanted follicles, 80-90% formed follicle clusters, indicative of the presence of transplanted follicles not containing colony-forming cells (clonogens) or some reproducible degree of transplantation trauma. The initial number of clonogens per regenerative follicle was calculated from data from split-dose experiments to be 3.3 +/- 1.5, and their sensitivity was characterized by a D0 value of 350 +/- 95 cGy. Three clonogens among about 80 epithelial cells per regenerative follicle and the 10-20% of nonregenerative follicles represent an overall colony-forming efficiency of about 3%. This is similar to the value of 2-3% reported by others using single-cell transplantation techniques.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Survival-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshFemale-
dc.subject.meshRadiation Tolerance-
dc.subject.meshRats-
dc.subject.meshRats, Inbred Strains-
dc.subject.meshStem Cells-
dc.subject.meshThymus Gland-
dc.titleClonogen number and radiosensitivity in rat thyroid follicles.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.en
dc.identifier.journalRadiation Researchen
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