Transcription of a stem/loop region of a tumour-amplified sequence induces bacterial aggregation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104733
Title:
Transcription of a stem/loop region of a tumour-amplified sequence induces bacterial aggregation.
Authors:
Heighway, Jim; Leverton, Kate E
Abstract:
A sequence of human DNA, amplified in a lung tumour, was shown to induce expression-dependent effects on Escherichia coli phenotype. Previous studies have demonstrated the induction of abnormal plasmid supercoiling and bacterial aggregation in host strains harbouring various constructs encoding this region. Subcloning identified a short stretch of 50 bp crucial for these effects. This study details further characterization of the active sequence. Computer analysis of the region predicted the formation of a stem/loop structure in transcribed RNA. Transcription, in the absence of translation, of a 22 bp sequence comprising this structure was sufficient to induce bacterial aggregation. Site-directed and random mutagenesis of the sub-region were carried out in order to identify critical factors in the induction of this phenotypic shift. It was found that changes in the loop sequence modulated activity, and mutations increasing predicted stem stability produced more active constructs. The wild-type sequence induced high level aggregation in only a limited number of strains but using the mutagenesis data, a sequence was synthesized that induced high level aggregation in most E. coli strains tested.
Affiliation:
CRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
Citation:
Transcription of a stem/loop region of a tumour-amplified sequence induces bacterial aggregation. 1991, 137 (10):2313-9 J. Gen. Microbiol.
Journal:
Journal of General Microbiology
Issue Date:
Oct-1991
URI:
http://hdl.handle.net/10541/104733
PubMed ID:
1770348
Type:
Article
Language:
en
ISSN:
0022-1287
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHeighway, Jimen
dc.contributor.authorLeverton, Kate Een
dc.date.accessioned2010-06-11T14:48:52Z-
dc.date.available2010-06-11T14:48:52Z-
dc.date.issued1991-10-
dc.identifier.citationTranscription of a stem/loop region of a tumour-amplified sequence induces bacterial aggregation. 1991, 137 (10):2313-9 J. Gen. Microbiol.en
dc.identifier.issn0022-1287-
dc.identifier.pmid1770348-
dc.identifier.urihttp://hdl.handle.net/10541/104733-
dc.description.abstractA sequence of human DNA, amplified in a lung tumour, was shown to induce expression-dependent effects on Escherichia coli phenotype. Previous studies have demonstrated the induction of abnormal plasmid supercoiling and bacterial aggregation in host strains harbouring various constructs encoding this region. Subcloning identified a short stretch of 50 bp crucial for these effects. This study details further characterization of the active sequence. Computer analysis of the region predicted the formation of a stem/loop structure in transcribed RNA. Transcription, in the absence of translation, of a 22 bp sequence comprising this structure was sufficient to induce bacterial aggregation. Site-directed and random mutagenesis of the sub-region were carried out in order to identify critical factors in the induction of this phenotypic shift. It was found that changes in the loop sequence modulated activity, and mutations increasing predicted stem stability produced more active constructs. The wild-type sequence induced high level aggregation in only a limited number of strains but using the mutagenesis data, a sequence was synthesized that induced high level aggregation in most E. coli strains tested.en
dc.language.isoenen
dc.subjectCancer DNAen
dc.subjectCancer RNAen
dc.subjectLung Canceren
dc.subject.meshBacterial Adhesion-
dc.subject.meshBase Sequence-
dc.subject.meshCarcinoma, Squamous Cell-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshEscherichia coli-
dc.subject.meshHumans-
dc.subject.meshLung Neoplasms-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMutagenesis-
dc.subject.meshMutagenesis, Site-Directed-
dc.subject.meshNucleic Acid Conformation-
dc.subject.meshPhenotype-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshRNA, Neoplasm-
dc.subject.meshStructure-Activity Relationship-
dc.subject.meshTranscription, Genetic-
dc.titleTranscription of a stem/loop region of a tumour-amplified sequence induces bacterial aggregation.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.en
dc.identifier.journalJournal of General Microbiologyen
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