Serum-free culture of enriched murine haemopoietic stem cells. II: Effects of growth factors and haemin on development.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104713
Title:
Serum-free culture of enriched murine haemopoietic stem cells. II: Effects of growth factors and haemin on development.
Authors:
Ponting, Ian L; Heyworth, Clare M; Cormier, F; Dexter, T Michael
Abstract:
A serum-free culture system was used to determine the effects of growth factors on the clonogenic development of a population of cells highly enriched for multipotential day 12 spleen colony forming cells (CFU-S) (FACS-BM). Under these conditions, interleukin-3 (IL-3) was found to be primarily a proliferative stimulus, the progenitor cells developing in the clonal assay systems produced colonies of morphologically undifferentiated cells for up to 20 days. No such induction of proliferation without maturation was observed with other growth factors (eg. granulocyte-macrophage colony stimulating factor (GM-CSF)). However, combinations of IL-3 plus secondary growth factors such as GM-CSF, macrophage colony stimulating factor (M-CSF), granulocyte colony-stimulating factor (G-CSF) or interleukin-1 (IL-1) led to the formation of colonies containing mature haemopoietic cells of the granulocytic, megakaryocytic or monocytic lineages. In contrast, erythroid development did not occur unless the protoporphyrin, haemin, was added to the cultures. Under these conditions mature erythroid cells were produced in cultures containing either IL-3 or GM-CSF (with or without erythropoietin (epo)). In replating experiments it was determined that the FACS-BM cells were able to generate large numbers of clonogenic cells for up to 30-40 free cultures. Such cultures, therefore, may be useful for investigating the biological and basis of the generation of clonogenic cells and of haemopoietic cell differentiation and development in response to growth factors.
Affiliation:
Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, U.K.
Citation:
Serum-free culture of enriched murine haemopoietic stem cells. II: Effects of growth factors and haemin on development. 1991, 4 (3):165-73 Growth Factors
Journal:
Growth Factors
Issue Date:
1991
URI:
http://hdl.handle.net/10541/104713
DOI:
10.3109/08977199109104812
PubMed ID:
1768433
Type:
Article
Language:
en
ISSN:
0897-7194
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPonting, Ian Len
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorCormier, Fen
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-06-11T11:31:49Z-
dc.date.available2010-06-11T11:31:49Z-
dc.date.issued1991-
dc.identifier.citationSerum-free culture of enriched murine haemopoietic stem cells. II: Effects of growth factors and haemin on development. 1991, 4 (3):165-73 Growth Factorsen
dc.identifier.issn0897-7194-
dc.identifier.pmid1768433-
dc.identifier.doi10.3109/08977199109104812-
dc.identifier.urihttp://hdl.handle.net/10541/104713-
dc.description.abstractA serum-free culture system was used to determine the effects of growth factors on the clonogenic development of a population of cells highly enriched for multipotential day 12 spleen colony forming cells (CFU-S) (FACS-BM). Under these conditions, interleukin-3 (IL-3) was found to be primarily a proliferative stimulus, the progenitor cells developing in the clonal assay systems produced colonies of morphologically undifferentiated cells for up to 20 days. No such induction of proliferation without maturation was observed with other growth factors (eg. granulocyte-macrophage colony stimulating factor (GM-CSF)). However, combinations of IL-3 plus secondary growth factors such as GM-CSF, macrophage colony stimulating factor (M-CSF), granulocyte colony-stimulating factor (G-CSF) or interleukin-1 (IL-1) led to the formation of colonies containing mature haemopoietic cells of the granulocytic, megakaryocytic or monocytic lineages. In contrast, erythroid development did not occur unless the protoporphyrin, haemin, was added to the cultures. Under these conditions mature erythroid cells were produced in cultures containing either IL-3 or GM-CSF (with or without erythropoietin (epo)). In replating experiments it was determined that the FACS-BM cells were able to generate large numbers of clonogenic cells for up to 30-40 free cultures. Such cultures, therefore, may be useful for investigating the biological and basis of the generation of clonogenic cells and of haemopoietic cell differentiation and development in response to growth factors.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshBone Marrow-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshCulture Media, Serum-Free-
dc.subject.meshErythropoietin-
dc.subject.meshFemale-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshGrowth Substances-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHemin-
dc.subject.meshInterleukin-3-
dc.subject.meshMacrophage Colony-Stimulating Factor-
dc.subject.meshMice-
dc.titleSerum-free culture of enriched murine haemopoietic stem cells. II: Effects of growth factors and haemin on development.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, U.K.en
dc.identifier.journalGrowth Factorsen
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