2.50
Hdl Handle:
http://hdl.handle.net/10541/104701
Title:
A serum-free medium for the Courtenay-Mills soft agar assay.
Authors:
Wilks, Deepti P; West, Catharine M L
Abstract:
A serum-free medium has been developed to support the clonogenic growth in soft agar of human cervical carcinoma cell lines xenografted into nude mice. Using the Courtenay-Mills assay, colony-forming efficiencies and colony sizes equivalent to those obtained using Ham's F12 plus 15% fetal calf serum can be obtained. Validation of the assay using the developed medium was obtained through establishing linearity between the number of cells plated and colonies formed, and by producing radiation survival curves.
Affiliation:
Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, United Kingdom.
Citation:
A serum-free medium for the Courtenay-Mills soft agar assay. 1991, 9 (6):559-69 Int. J. Cell Cloning
Journal:
International Journal of Cell Cloning
Issue Date:
Nov-1991
URI:
http://hdl.handle.net/10541/104701
DOI:
10.1002/stem.5530090606
PubMed ID:
1770230
Type:
Article
Language:
en
ISSN:
0737-1454
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWilks, Deepti Pen
dc.contributor.authorWest, Catharine M Len
dc.date.accessioned2010-06-11T11:42:37Z-
dc.date.available2010-06-11T11:42:37Z-
dc.date.issued1991-11-
dc.identifier.citationA serum-free medium for the Courtenay-Mills soft agar assay. 1991, 9 (6):559-69 Int. J. Cell Cloningen
dc.identifier.issn0737-1454-
dc.identifier.pmid1770230-
dc.identifier.doi10.1002/stem.5530090606-
dc.identifier.urihttp://hdl.handle.net/10541/104701-
dc.description.abstractA serum-free medium has been developed to support the clonogenic growth in soft agar of human cervical carcinoma cell lines xenografted into nude mice. Using the Courtenay-Mills assay, colony-forming efficiencies and colony sizes equivalent to those obtained using Ham's F12 plus 15% fetal calf serum can be obtained. Validation of the assay using the developed medium was obtained through establishing linearity between the number of cells plated and colonies formed, and by producing radiation survival curves.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subjectTumour Stem Cell Assayen
dc.subject.meshAgar-
dc.subject.meshAnimals-
dc.subject.meshCell Survival-
dc.subject.meshClone Cells-
dc.subject.meshCulture Media, Serum-Free-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Nude-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshTumor Stem Cell Assay-
dc.titleA serum-free medium for the Courtenay-Mills soft agar assay.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, United Kingdom.en
dc.identifier.journalInternational Journal of Cell Cloningen
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