The radiosensitivity of populations of murine hemopoietic colony-forming cells that respond to combinations of growth factors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104695
Title:
The radiosensitivity of populations of murine hemopoietic colony-forming cells that respond to combinations of growth factors.
Authors:
Baird, M C; Hendry, Jolyon H; Dexter, T Michael; Testa, Nydia G
Abstract:
Combinations of murine recombinant interleukin 3 (IL-3), purified murine macrophage colony-stimulating factor (M-CSF), and human recombinant interleukin 1 alpha (IL-1 alpha) were used to determine the effects of growth factors on the measured radiosensitivity of different populations of murine colony-forming cells (CFC). The data showed that combinations of growth factors resulted in different values of CFC radiosensitivity, being less than values observed when colony growth was stimulated using a single factor. For various combinations of growth factors, Do values ranged from 106 +/- 8 to 175 +/- 24 cGy for progenitor cells in normal bone marrow; 74 +/- 3 to 171 +/- 18 cGy for primitive multipotent CFC enriched using fluorescence-activated cell sorting; and from 46 +/- 4 to 131 +/- 10 cGy for more mature granulocyte-macrophage CFC, enriched by counterflow centrifugal elutriation. Only combinations of three factors produced the high values of Do reported in experiments using unpurified conditioned medium as a stimulus for colony formation.
Affiliation:
Cancer Research Campaign Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
Citation:
The radiosensitivity of populations of murine hemopoietic colony-forming cells that respond to combinations of growth factors. 1991, 19 (4):282-7 Exp. Hematol.
Journal:
Experimental Hematology
Issue Date:
May-1991
URI:
http://hdl.handle.net/10541/104695
PubMed ID:
2055293
Type:
Article
Language:
en
ISSN:
0301-472X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBaird, M Cen
dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-06-11T10:45:51Z-
dc.date.available2010-06-11T10:45:51Z-
dc.date.issued1991-05-
dc.identifier.citationThe radiosensitivity of populations of murine hemopoietic colony-forming cells that respond to combinations of growth factors. 1991, 19 (4):282-7 Exp. Hematol.en
dc.identifier.issn0301-472X-
dc.identifier.pmid2055293-
dc.identifier.urihttp://hdl.handle.net/10541/104695-
dc.description.abstractCombinations of murine recombinant interleukin 3 (IL-3), purified murine macrophage colony-stimulating factor (M-CSF), and human recombinant interleukin 1 alpha (IL-1 alpha) were used to determine the effects of growth factors on the measured radiosensitivity of different populations of murine colony-forming cells (CFC). The data showed that combinations of growth factors resulted in different values of CFC radiosensitivity, being less than values observed when colony growth was stimulated using a single factor. For various combinations of growth factors, Do values ranged from 106 +/- 8 to 175 +/- 24 cGy for progenitor cells in normal bone marrow; 74 +/- 3 to 171 +/- 18 cGy for primitive multipotent CFC enriched using fluorescence-activated cell sorting; and from 46 +/- 4 to 131 +/- 10 cGy for more mature granulocyte-macrophage CFC, enriched by counterflow centrifugal elutriation. Only combinations of three factors produced the high values of Do reported in experiments using unpurified conditioned medium as a stimulus for colony formation.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBone Marrow-
dc.subject.meshDrug Combinations-
dc.subject.meshFemale-
dc.subject.meshFlow Cytometry-
dc.subject.meshInterleukin-3-
dc.subject.meshMacrophage Colony-Stimulating Factor-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshRadiation Tolerance-
dc.subject.meshStem Cells-
dc.titleThe radiosensitivity of populations of murine hemopoietic colony-forming cells that respond to combinations of growth factors.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalExperimental Hematologyen

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