Retroviral vectors related to the myeloproliferative sarcoma virus allow efficient expression in hematopoietic stem and precursor cell lines, but retroviral infection is reduced in more primitive cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104684
Title:
Retroviral vectors related to the myeloproliferative sarcoma virus allow efficient expression in hematopoietic stem and precursor cell lines, but retroviral infection is reduced in more primitive cells.
Authors:
Beck-Engeser, G; Stocking, C; Just, U; Albritton, L; Dexter, T Michael; Spooncer, Elaine; Ostertag, W
Abstract:
Retroviral vectors are considered to be the most suited vehicles for somatic gene therapy with hematopoietic stem cells as targets. Retrovirus-mediated gene transfer into differentiation-restricted hematopoietic precursor (FDC-P1, FDC-P2) and multipotent progenitor (stem) cell lines (FDC-Pmix) is inefficient. Two cellular restrictions are involved. One is specific for stem but not precursor cells and is at the level of transcription. Due to a unique property of the transcriptional control region of the myeloproliferative sarcoma virus (MPSV), vectors derived from MPSV are not affected by this block. The second restriction occurs before proviral DNA synthesis and integration. This inhibition of effective viral infection depends on the state of differentiation, being more pronounced in multipotent clonogenic blast cells. This block to retroviral infection affects all retroviral vectors tested.
Affiliation:
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.
Citation:
Retroviral vectors related to the myeloproliferative sarcoma virus allow efficient expression in hematopoietic stem and precursor cell lines, but retroviral infection is reduced in more primitive cells. 1991, 2 (1):61-70 Hum. Gene Ther.
Journal:
Human Gene Therapy
Issue Date:
1991
URI:
http://hdl.handle.net/10541/104684
DOI:
10.1089/hum.1991.2.1-61
PubMed ID:
1863641
Type:
Article
Language:
en
ISSN:
1043-0342
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBeck-Engeser, Gen
dc.contributor.authorStocking, Cen
dc.contributor.authorJust, Uen
dc.contributor.authorAlbritton, Len
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorSpooncer, Elaineen
dc.contributor.authorOstertag, Wen
dc.date.accessioned2010-06-11T10:48:39Z-
dc.date.available2010-06-11T10:48:39Z-
dc.date.issued1991-
dc.identifier.citationRetroviral vectors related to the myeloproliferative sarcoma virus allow efficient expression in hematopoietic stem and precursor cell lines, but retroviral infection is reduced in more primitive cells. 1991, 2 (1):61-70 Hum. Gene Ther.en
dc.identifier.issn1043-0342-
dc.identifier.pmid1863641-
dc.identifier.doi10.1089/hum.1991.2.1-61-
dc.identifier.urihttp://hdl.handle.net/10541/104684-
dc.description.abstractRetroviral vectors are considered to be the most suited vehicles for somatic gene therapy with hematopoietic stem cells as targets. Retrovirus-mediated gene transfer into differentiation-restricted hematopoietic precursor (FDC-P1, FDC-P2) and multipotent progenitor (stem) cell lines (FDC-Pmix) is inefficient. Two cellular restrictions are involved. One is specific for stem but not precursor cells and is at the level of transcription. Due to a unique property of the transcriptional control region of the myeloproliferative sarcoma virus (MPSV), vectors derived from MPSV are not affected by this block. The second restriction occurs before proviral DNA synthesis and integration. This inhibition of effective viral infection depends on the state of differentiation, being more pronounced in multipotent clonogenic blast cells. This block to retroviral infection affects all retroviral vectors tested.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Line-
dc.subject.meshDNA, Viral-
dc.subject.meshDefective Viruses-
dc.subject.meshFibroblasts-
dc.subject.meshGene Expression Regulation-
dc.subject.meshGenetic Vectors-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshMice-
dc.subject.meshMoloney murine sarcoma virus-
dc.subject.meshProviruses-
dc.subject.meshReceptors, Virus-
dc.subject.meshTranscription, Genetic-
dc.subject.meshTransfection-
dc.subject.meshTransformation, Genetic-
dc.titleRetroviral vectors related to the myeloproliferative sarcoma virus allow efficient expression in hematopoietic stem and precursor cell lines, but retroviral infection is reduced in more primitive cells.en
dc.typeArticleen
dc.contributor.departmentHeinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.en
dc.identifier.journalHuman Gene Therapyen

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