Interferon-gamma stimulates the survival and influences the development of bipotential granulocyte-macrophage colony-forming cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104672
Title:
Interferon-gamma stimulates the survival and influences the development of bipotential granulocyte-macrophage colony-forming cells.
Authors:
Kan, O; Heyworth, Clare M; Dexter, T Michael; Maudsley, P; Cook, N; Vallance, S; Whetton, Anthony D
Abstract:
The effects of interferon-gamma (IFN-gamma) on a highly enriched population of granulocyte-macrophage colony-forming cells (GM-CFC) were assessed. When added with myeloid growth factors (interleukin-3 [IL-3], granulocyte-macrophage colony-stimulating factor [GM-CSF], or macrophage-CSF [M-CSF]), IFN-gamma inhibited the formation of colonies in soft agar assays. Furthermore IFN-gamma stimulated an increase in the number of macrophages present in colonies formed in the presence of IL-3. IFN-gamma also inhibited M-CSF-, GM-CSF-, or IL-3-stimulated [3H]-thymidine incorporation in highly enriched GM-CFC. However, when added in the absence of hematopoietic growth factors, IFN-gamma promoted the survival of GM-CFC and had a modest stimulatory effect on DNA synthesis. The direct interaction of the IFN with GM-CFC was confirmed by showing its ability to rapidly activate the sodium/hydrogen antiport in GM-CFC, as do the mitogens GM-CSF, M-CSF, and IL-3. However, the effect of IFN-gamma on intracellular pH and DNA synthesis was transient and pretreatment with IFN markedly inhibited the ability of GM-CSF, M-CSF, and IL-3 to activate the sodium/hydrogen antiport. IFN-gamma has a dual effect on GM-CFC, decreasing the rate of cell death but also limiting the proliferative response to CSFs.
Affiliation:
Department of Biochemistry and Applied Molecular Biology, UMIST, Manchester, UK.
Citation:
Interferon-gamma stimulates the survival and influences the development of bipotential granulocyte-macrophage colony-forming cells. 1991, 78 (10):2588-94 Blood
Journal:
Blood
Issue Date:
15-Nov-1991
URI:
http://hdl.handle.net/10541/104672
PubMed ID:
1824254
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorKan, Oen
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorMaudsley, Pen
dc.contributor.authorCook, Nen
dc.contributor.authorVallance, Sen
dc.contributor.authorWhetton, Anthony Den
dc.date.accessioned2010-06-11T10:37:40Z-
dc.date.available2010-06-11T10:37:40Z-
dc.date.issued1991-11-15-
dc.identifier.citationInterferon-gamma stimulates the survival and influences the development of bipotential granulocyte-macrophage colony-forming cells. 1991, 78 (10):2588-94 Blooden
dc.identifier.issn0006-4971-
dc.identifier.pmid1824254-
dc.identifier.urihttp://hdl.handle.net/10541/104672-
dc.description.abstractThe effects of interferon-gamma (IFN-gamma) on a highly enriched population of granulocyte-macrophage colony-forming cells (GM-CFC) were assessed. When added with myeloid growth factors (interleukin-3 [IL-3], granulocyte-macrophage colony-stimulating factor [GM-CSF], or macrophage-CSF [M-CSF]), IFN-gamma inhibited the formation of colonies in soft agar assays. Furthermore IFN-gamma stimulated an increase in the number of macrophages present in colonies formed in the presence of IL-3. IFN-gamma also inhibited M-CSF-, GM-CSF-, or IL-3-stimulated [3H]-thymidine incorporation in highly enriched GM-CFC. However, when added in the absence of hematopoietic growth factors, IFN-gamma promoted the survival of GM-CFC and had a modest stimulatory effect on DNA synthesis. The direct interaction of the IFN with GM-CFC was confirmed by showing its ability to rapidly activate the sodium/hydrogen antiport in GM-CFC, as do the mitogens GM-CSF, M-CSF, and IL-3. However, the effect of IFN-gamma on intracellular pH and DNA synthesis was transient and pretreatment with IFN markedly inhibited the ability of GM-CSF, M-CSF, and IL-3 to activate the sodium/hydrogen antiport. IFN-gamma has a dual effect on GM-CFC, decreasing the rate of cell death but also limiting the proliferative response to CSFs.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshBone Marrow Cells-
dc.subject.meshCell Survival-
dc.subject.meshCells, Cultured-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshDNA Replication-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshGranulocytes-
dc.subject.meshGrowth Substances-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHydrogen-Ion Concentration-
dc.subject.meshInterferon-gamma, Recombinant-
dc.subject.meshInterleukin-3-
dc.subject.meshKinetics-
dc.subject.meshMacrophage Colony-Stimulating Factor-
dc.subject.meshMacrophages-
dc.subject.meshMice-
dc.subject.meshRecombinant Proteins-
dc.subject.meshTime Factors-
dc.titleInterferon-gamma stimulates the survival and influences the development of bipotential granulocyte-macrophage colony-forming cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biochemistry and Applied Molecular Biology, UMIST, Manchester, UK.en
dc.identifier.journalBlooden
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