The detection of alkylation damage in the DNA of human gastrointestinal tissues.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104661
Title:
The detection of alkylation damage in the DNA of human gastrointestinal tissues.
Authors:
Hall, C N; Badawi, A F; O'Connor, Peter J; Saffhill, Roy
Abstract:
Damage arising from putative environmental sources has been found in the DNA of the gastric and colorectal mucosae of patients presenting with gastrointestinal disorders from the South Manchester area. O6-Methylguanine (O6-MeG) in the range 0.010- greater than 0.300 mu moles mole-1 adenine was heterogeneously distributed both between and within individuals. The pattern of alkylation of tissue DNA appears to differ when comparison is made between gastric and colorectal samples. Most of the gastric tumour DNA samples were alkylated (5/6; 0.087 +/- 0.097), whereas the DNA of the associated mucosa was alkylated less frequently (2/7) and to a lesser extent; (0.017 +/- 0.030; P = 0.07). Conversely, colorectal tumour DNA was alkylated infrequently (1/7) and to a lower extent (0.003 +/- 0.007) than the DNA of the adjacent mucosa (8/10 samples alkylated with a mean of 0.083 +/- 0.106; P = less than 0.01), or indeed of any other tissue. Although increased levels of DNA damage in tissue associated with malignant disease have been indicated by independent studies of DNA damage at other cancer sites, significant differences were not observed in the present report, neither was there any suggestion of a relationship with smoking or alcohol consumption. The data provided by this report indicate that exposure to putative environmental alkylating agents occurs in the UK at levels comparable to those previously detected in areas of higher cancer risk. Although we cannot determine the extent to which this DNA damage is attributable to normal background exposures, it is evident that the alkylation of tissue DNA occurs and is not uniform. In conjunction with other reports, therefore these differences may begin to provide indications of mechanisms that could be of relevance in the aetiology of gastrointestinal cancers.
Affiliation:
Department of Surgery, Wythenshawe Hospital, Manchester.
Citation:
The detection of alkylation damage in the DNA of human gastrointestinal tissues. 1991, 64 (1):59-63 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Jul-1991
URI:
http://hdl.handle.net/10541/104661
PubMed ID:
1854628
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHall, C Nen
dc.contributor.authorBadawi, A Fen
dc.contributor.authorO'Connor, Peter Jen
dc.contributor.authorSaffhill, Royen
dc.date.accessioned2010-06-11T09:06:18Z-
dc.date.available2010-06-11T09:06:18Z-
dc.date.issued1991-07-
dc.identifier.citationThe detection of alkylation damage in the DNA of human gastrointestinal tissues. 1991, 64 (1):59-63 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid1854628-
dc.identifier.urihttp://hdl.handle.net/10541/104661-
dc.description.abstractDamage arising from putative environmental sources has been found in the DNA of the gastric and colorectal mucosae of patients presenting with gastrointestinal disorders from the South Manchester area. O6-Methylguanine (O6-MeG) in the range 0.010- greater than 0.300 mu moles mole-1 adenine was heterogeneously distributed both between and within individuals. The pattern of alkylation of tissue DNA appears to differ when comparison is made between gastric and colorectal samples. Most of the gastric tumour DNA samples were alkylated (5/6; 0.087 +/- 0.097), whereas the DNA of the associated mucosa was alkylated less frequently (2/7) and to a lesser extent; (0.017 +/- 0.030; P = 0.07). Conversely, colorectal tumour DNA was alkylated infrequently (1/7) and to a lower extent (0.003 +/- 0.007) than the DNA of the adjacent mucosa (8/10 samples alkylated with a mean of 0.083 +/- 0.106; P = less than 0.01), or indeed of any other tissue. Although increased levels of DNA damage in tissue associated with malignant disease have been indicated by independent studies of DNA damage at other cancer sites, significant differences were not observed in the present report, neither was there any suggestion of a relationship with smoking or alcohol consumption. The data provided by this report indicate that exposure to putative environmental alkylating agents occurs in the UK at levels comparable to those previously detected in areas of higher cancer risk. Although we cannot determine the extent to which this DNA damage is attributable to normal background exposures, it is evident that the alkylation of tissue DNA occurs and is not uniform. In conjunction with other reports, therefore these differences may begin to provide indications of mechanisms that could be of relevance in the aetiology of gastrointestinal cancers.en
dc.language.isoenen
dc.subjectGastrointestinal Canceren
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAlkylation-
dc.subject.meshDNA Damage-
dc.subject.meshGastric Mucosa-
dc.subject.meshGastrointestinal Diseases-
dc.subject.meshGastrointestinal Neoplasms-
dc.subject.meshHumans-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshIntestines-
dc.subject.meshLife Style-
dc.subject.meshMiddle Aged-
dc.subject.meshStomach-
dc.titleThe detection of alkylation damage in the DNA of human gastrointestinal tissues.en
dc.typeArticleen
dc.contributor.departmentDepartment of Surgery, Wythenshawe Hospital, Manchester.en
dc.identifier.journalBritish Journal of Canceren
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