Expression of the GM-CSF gene after retroviral transfer in hematopoietic stem cell lines induces synchronous granulocyte-macrophage differentiation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104658
Title:
Expression of the GM-CSF gene after retroviral transfer in hematopoietic stem cell lines induces synchronous granulocyte-macrophage differentiation.
Authors:
Just, U; Stocking, C; Spooncer, Elaine; Dexter, T Michael; Ostertag, W
Abstract:
Multipotent murine stem cell lines (FDC-Pmix) depend on IL-3 for self-renewal and proliferation and can be induced to differentiate into multiple hematopoietic lineages. Single FDC-Pmix cells infected with retroviral vectors expressing GM-CSF are induced to differentiate into granulocytes and macrophages. This results in a complete loss of clonogenic cells if IL-3 is not exogenously supplied; however, multipotent variants can be selected that do not terminally differentiate if cells are kept in the presence of IL-3. Unidirectional and synchronous granulocyte and macrophage differentiation accompanied with loss of self-renewal capacity is induced when IL-3 is removed. Our data indicate that activation of the GM-CSF receptor induces differentiation of stem cells by an instructive mechanism that can be blocked by the activated IL-3 receptor. A model of how receptors can induce proliferation and cell-specific differentiation by two separate pathways is discussed.
Affiliation:
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.
Citation:
Expression of the GM-CSF gene after retroviral transfer in hematopoietic stem cell lines induces synchronous granulocyte-macrophage differentiation. 1991, 64 (6):1163-73 Cell
Journal:
Cell
Issue Date:
22-Mar-1991
URI:
http://hdl.handle.net/10541/104658
DOI:
10.1016/0092-8674(91)90271-Y
PubMed ID:
2004422
Type:
Article
Language:
en
ISSN:
0092-8674
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorJust, Uen
dc.contributor.authorStocking, Cen
dc.contributor.authorSpooncer, Elaineen
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorOstertag, Wen
dc.date.accessioned2010-06-11T08:58:19Z-
dc.date.available2010-06-11T08:58:19Z-
dc.date.issued1991-03-22-
dc.identifier.citationExpression of the GM-CSF gene after retroviral transfer in hematopoietic stem cell lines induces synchronous granulocyte-macrophage differentiation. 1991, 64 (6):1163-73 Cellen
dc.identifier.issn0092-8674-
dc.identifier.pmid2004422-
dc.identifier.doi10.1016/0092-8674(91)90271-Y-
dc.identifier.urihttp://hdl.handle.net/10541/104658-
dc.description.abstractMultipotent murine stem cell lines (FDC-Pmix) depend on IL-3 for self-renewal and proliferation and can be induced to differentiate into multiple hematopoietic lineages. Single FDC-Pmix cells infected with retroviral vectors expressing GM-CSF are induced to differentiate into granulocytes and macrophages. This results in a complete loss of clonogenic cells if IL-3 is not exogenously supplied; however, multipotent variants can be selected that do not terminally differentiate if cells are kept in the presence of IL-3. Unidirectional and synchronous granulocyte and macrophage differentiation accompanied with loss of self-renewal capacity is induced when IL-3 is removed. Our data indicate that activation of the GM-CSF receptor induces differentiation of stem cells by an instructive mechanism that can be blocked by the activated IL-3 receptor. A model of how receptors can induce proliferation and cell-specific differentiation by two separate pathways is discussed.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Division-
dc.subject.meshCell Line-
dc.subject.meshGenetic Vectors-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshGranulocytes-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshInterleukin-3-
dc.subject.meshMacrophages-
dc.subject.meshMice-
dc.subject.meshMutation-
dc.subject.meshRetroviridae-
dc.titleExpression of the GM-CSF gene after retroviral transfer in hematopoietic stem cell lines induces synchronous granulocyte-macrophage differentiation.en
dc.typeArticleen
dc.contributor.departmentHeinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.en
dc.identifier.journalCellen
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