Assessment of P-glycoprotein, glutathione-based detoxifying enzymes and O6-alkylguanine-DNA alkyltransferase as potential indicators of constitutive drug resistance in human colorectal tumors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104657
Title:
Assessment of P-glycoprotein, glutathione-based detoxifying enzymes and O6-alkylguanine-DNA alkyltransferase as potential indicators of constitutive drug resistance in human colorectal tumors.
Authors:
Redmond, S M; Joncourt, F; Buser, K; Ziemiecki, A; Altermatt, H J; Fey, M; Margison, Geoffrey P; Cerny, T
Abstract:
Drug resistance is a major problem in cancer chemotherapy. Treatment protocols generally include a number of different cytotoxic drugs given in combination. Therefore, drug resistance in the tumor is likely to result from the coexpression of several cellular activities able to prevent cell killing by any of the drugs used. In this study we have measured several potential drug resistance mechanisms consisting of the multidrug resistance gene product P-glycoprotein, glutathione, glutathione-transferase and -peroxidase, and the DNA repair enzyme O6-alkylguanine-DNA-alkyltransferase in samples of colon carcinoma and normal adjacent mucosa from 23 untreated patients. All of these, with the exception of P-glycoprotein, showed significant increases in tumor tissue levels when compared with normal tissue from the same patient. The significance was highest for glutathione peroxidase (P less than or equal to 0.0005). Individual patients, however, showed very different patterns, with none, several, or all monitored resistance mechanisms elevated in the tumor. The implications both in the choice of drugs and in the use of resistance modifying agents to improve therapy for the individual patient are discussed.
Affiliation:
Institute for Clinical & Experimental Cancer Research, Berne, Switzerland.
Citation:
Assessment of P-glycoprotein, glutathione-based detoxifying enzymes and O6-alkylguanine-DNA alkyltransferase as potential indicators of constitutive drug resistance in human colorectal tumors. 1991, 51 (8):2092-7 Cancer Res.
Journal:
Cancer Research
Issue Date:
15-Apr-1991
URI:
http://hdl.handle.net/10541/104657
PubMed ID:
1672623
Type:
Article
Language:
en
ISSN:
0008-5472
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorRedmond, S Men
dc.contributor.authorJoncourt, Fen
dc.contributor.authorBuser, Ken
dc.contributor.authorZiemiecki, Aen
dc.contributor.authorAltermatt, H Jen
dc.contributor.authorFey, Men
dc.contributor.authorMargison, Geoffrey Pen
dc.contributor.authorCerny, Ten
dc.date.accessioned2010-06-11T08:49:20Z-
dc.date.available2010-06-11T08:49:20Z-
dc.date.issued1991-04-15-
dc.identifier.citationAssessment of P-glycoprotein, glutathione-based detoxifying enzymes and O6-alkylguanine-DNA alkyltransferase as potential indicators of constitutive drug resistance in human colorectal tumors. 1991, 51 (8):2092-7 Cancer Res.en
dc.identifier.issn0008-5472-
dc.identifier.pmid1672623-
dc.identifier.urihttp://hdl.handle.net/10541/104657-
dc.description.abstractDrug resistance is a major problem in cancer chemotherapy. Treatment protocols generally include a number of different cytotoxic drugs given in combination. Therefore, drug resistance in the tumor is likely to result from the coexpression of several cellular activities able to prevent cell killing by any of the drugs used. In this study we have measured several potential drug resistance mechanisms consisting of the multidrug resistance gene product P-glycoprotein, glutathione, glutathione-transferase and -peroxidase, and the DNA repair enzyme O6-alkylguanine-DNA-alkyltransferase in samples of colon carcinoma and normal adjacent mucosa from 23 untreated patients. All of these, with the exception of P-glycoprotein, showed significant increases in tumor tissue levels when compared with normal tissue from the same patient. The significance was highest for glutathione peroxidase (P less than or equal to 0.0005). Individual patients, however, showed very different patterns, with none, several, or all monitored resistance mechanisms elevated in the tumor. The implications both in the choice of drugs and in the use of resistance modifying agents to improve therapy for the individual patient are discussed.en
dc.language.isoenen
dc.subjectColonic Canceren
dc.subjectBiological Tumour Markersen
dc.subject.meshAged-
dc.subject.meshBacterial Proteins-
dc.subject.meshColonic Neoplasms-
dc.subject.meshDrug Resistance-
dc.subject.meshEscherichia coli Proteins-
dc.subject.meshFemale-
dc.subject.meshGlutathione-
dc.subject.meshGlutathione Peroxidase-
dc.subject.meshGlutathione Transferase-
dc.subject.meshHumans-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshMale-
dc.subject.meshMembrane Glycoproteins-
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase-
dc.subject.meshP-Glycoprotein-
dc.subject.meshPilot Projects-
dc.subject.meshTranscription Factors-
dc.subject.meshTumor Markers, Biological-
dc.titleAssessment of P-glycoprotein, glutathione-based detoxifying enzymes and O6-alkylguanine-DNA alkyltransferase as potential indicators of constitutive drug resistance in human colorectal tumors.en
dc.typeArticleen
dc.contributor.departmentInstitute for Clinical & Experimental Cancer Research, Berne, Switzerland.en
dc.identifier.journalCancer Researchen

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