In vitro assessment of marrow 'stem cell' and stromal cell function in aplastic anaemia.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104607
Title:
In vitro assessment of marrow 'stem cell' and stromal cell function in aplastic anaemia.
Authors:
Marsh, Judith C W; Chang, James; Testa, Nydia G; Hows, J M; Dexter, T Michael
Abstract:
An in vitro model system is described that allows separate assessment of 'stem cell' and stromal cell function in aplastic anaemia (AA). Seven patients with non-severe AA, who had responded to immunosuppressive therapy and had haematological evidence of residual marrow function, were studied. Of these, three with otherwise typical AA had an acquired clonal cytogenetic marker. Purified bone marrow haemopoietic progenitors labelled with CD34 monoclonal antibody were positively selected using the fluorescence activated cell sorter (FACS) from both normal subjects and from patients with AA. The generative capacity of the CD34 positive cells was assessed by monitoring the output of granulocyte/macrophage colony forming cells (CFU-GM) in the non-adherent layer after inoculation onto irradiated performed long-term marrow culture (LTBMC) stromas. Stromal function in AA was assessed by inoculating CD34 positive cells from normal bone marrow onto performed irradiated stromas from patients with AA. Haemopoietic cell ('stem cell') function in AA was assessed by inoculating CD34 positive cells from AA patients onto confluent irradiated normal marrow stromas. Using these crossover/LTBMC experiments, all patients exhibited severe defects in haemopoietic cell function with normal functioning stroma. The proportion of CD34 positive cells present in bone marrow from these patients was reduced compared with controls, they comprised fewer small primitive 'blast-like' cells which in normal bone marrow are known to possess marrow repopulating ability, and demonstrated reduced clonogenic potential in short-term colony assays.
Affiliation:
Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital, Manchester.
Citation:
In vitro assessment of marrow 'stem cell' and stromal cell function in aplastic anaemia. 1991, 78 (2):258-67 Br. J. Haematol.
Journal:
British Journal of Haematology
Issue Date:
Jun-1991
URI:
http://hdl.handle.net/10541/104607
DOI:
10.1111/j.1365-2141.1991.tb04426.x
PubMed ID:
1712224
Type:
Article
Language:
en
ISSN:
0007-1048
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMarsh, Judith C Wen
dc.contributor.authorChang, Jamesen
dc.contributor.authorTesta, Nydia Gen
dc.contributor.authorHows, J Men
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-06-10T10:46:52Z-
dc.date.available2010-06-10T10:46:52Z-
dc.date.issued1991-06-
dc.identifier.citationIn vitro assessment of marrow 'stem cell' and stromal cell function in aplastic anaemia. 1991, 78 (2):258-67 Br. J. Haematol.en
dc.identifier.issn0007-1048-
dc.identifier.pmid1712224-
dc.identifier.doi10.1111/j.1365-2141.1991.tb04426.x-
dc.identifier.urihttp://hdl.handle.net/10541/104607-
dc.description.abstractAn in vitro model system is described that allows separate assessment of 'stem cell' and stromal cell function in aplastic anaemia (AA). Seven patients with non-severe AA, who had responded to immunosuppressive therapy and had haematological evidence of residual marrow function, were studied. Of these, three with otherwise typical AA had an acquired clonal cytogenetic marker. Purified bone marrow haemopoietic progenitors labelled with CD34 monoclonal antibody were positively selected using the fluorescence activated cell sorter (FACS) from both normal subjects and from patients with AA. The generative capacity of the CD34 positive cells was assessed by monitoring the output of granulocyte/macrophage colony forming cells (CFU-GM) in the non-adherent layer after inoculation onto irradiated performed long-term marrow culture (LTBMC) stromas. Stromal function in AA was assessed by inoculating CD34 positive cells from normal bone marrow onto performed irradiated stromas from patients with AA. Haemopoietic cell ('stem cell') function in AA was assessed by inoculating CD34 positive cells from AA patients onto confluent irradiated normal marrow stromas. Using these crossover/LTBMC experiments, all patients exhibited severe defects in haemopoietic cell function with normal functioning stroma. The proportion of CD34 positive cells present in bone marrow from these patients was reduced compared with controls, they comprised fewer small primitive 'blast-like' cells which in normal bone marrow are known to possess marrow repopulating ability, and demonstrated reduced clonogenic potential in short-term colony assays.en
dc.language.isoenen
dc.subjectAplastic Anaemiaen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAdult-
dc.subject.meshAnemia, Aplastic-
dc.subject.meshAntigens, CD-
dc.subject.meshAntigens, CD34-
dc.subject.meshBone Marrow-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshCulture Techniques-
dc.subject.meshFemale-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshModels, Biological-
dc.titleIn vitro assessment of marrow 'stem cell' and stromal cell function in aplastic anaemia.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital, Manchester.en
dc.identifier.journalBritish Journal of Haematologyen

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