DNA methylation in specific cells of rat liver by N-nitrosodimethylamine and N-nitrosomethylbenzylamine.

2.50
Hdl Handle:
http://hdl.handle.net/10541/104584
Title:
DNA methylation in specific cells of rat liver by N-nitrosodimethylamine and N-nitrosomethylbenzylamine.
Authors:
Dai, W D; Lee, V; Chin, W; Cooper, Donald P; Archer, M C; O'Connor, Peter J
Abstract:
Dose-response curves for the O6-methylation of guanine in the hepatic DNA of Wistar and Sprague-Dawley rats were determined after administration of N-nitrosomethylbenzylamine (NMBzA) or N-nitrosodimethylamine (NDMA). Similar results were obtained for both rat strains but methylation of hepatic DNA by NDMA was approximately 9-fold more efficient than with NMBzA when doses were compared on a molar basis. Comparison by immunohistochemical analysis of the distribution of nuclei containing O6-methylguanine within the liver lobules showed that both agents tended to alkylate cells close to the central veins at the lower doses. With increasing doses, the band width of alkylated cells around the central vein increased, spreading in the case of NDMA virtually into the portal zones, whereas with NMBzA the zone of alkylated nuclei reached little more than halfway from the central vein to the portal zone. These differences in the distribution of alkylated cells may explain the differing hepatic responses to these two nitrosamines.
Affiliation:
Cancer Research Campaign Section of Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
Citation:
DNA methylation in specific cells of rat liver by N-nitrosodimethylamine and N-nitrosomethylbenzylamine. 1991, 12 (7):1325-9 Carcinogenesis
Journal:
Carcinogenesis
Issue Date:
Jul-1991
URI:
http://hdl.handle.net/10541/104584
DOI:
10.1093/carcin/12.7.1325
PubMed ID:
2070499
Type:
Article
Language:
en
ISSN:
0143-3334
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorDai, W Den
dc.contributor.authorLee, Ven
dc.contributor.authorChin, Wen
dc.contributor.authorCooper, Donald Pen
dc.contributor.authorArcher, M Cen
dc.contributor.authorO'Connor, Peter Jen
dc.date.accessioned2010-06-10T09:04:57Z-
dc.date.available2010-06-10T09:04:57Z-
dc.date.issued1991-07-
dc.identifier.citationDNA methylation in specific cells of rat liver by N-nitrosodimethylamine and N-nitrosomethylbenzylamine. 1991, 12 (7):1325-9 Carcinogenesisen
dc.identifier.issn0143-3334-
dc.identifier.pmid2070499-
dc.identifier.doi10.1093/carcin/12.7.1325-
dc.identifier.urihttp://hdl.handle.net/10541/104584-
dc.description.abstractDose-response curves for the O6-methylation of guanine in the hepatic DNA of Wistar and Sprague-Dawley rats were determined after administration of N-nitrosomethylbenzylamine (NMBzA) or N-nitrosodimethylamine (NDMA). Similar results were obtained for both rat strains but methylation of hepatic DNA by NDMA was approximately 9-fold more efficient than with NMBzA when doses were compared on a molar basis. Comparison by immunohistochemical analysis of the distribution of nuclei containing O6-methylguanine within the liver lobules showed that both agents tended to alkylate cells close to the central veins at the lower doses. With increasing doses, the band width of alkylated cells around the central vein increased, spreading in the case of NDMA virtually into the portal zones, whereas with NMBzA the zone of alkylated nuclei reached little more than halfway from the central vein to the portal zone. These differences in the distribution of alkylated cells may explain the differing hepatic responses to these two nitrosamines.en
dc.language.isoenen
dc.subjectExperimental Liver Canceren
dc.subject.meshAnimals-
dc.subject.meshCarcinogens-
dc.subject.meshCytochrome P-450 Enzyme System-
dc.subject.meshDNA-
dc.subject.meshDimethylnitrosamine-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshGuanine-
dc.subject.meshLiver-
dc.subject.meshLiver Neoplasms, Experimental-
dc.subject.meshMale-
dc.subject.meshMethylation-
dc.subject.meshRats-
dc.subject.meshSpecies Specificity-
dc.titleDNA methylation in specific cells of rat liver by N-nitrosodimethylamine and N-nitrosomethylbenzylamine.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Section of Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.en
dc.identifier.journalCarcinogenesisen
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