The differentiation and lineage development of goblet cells in the murine small intestinal crypt: experimental and modelling studies.

2.50
Hdl Handle:
http://hdl.handle.net/10541/101876
Title:
The differentiation and lineage development of goblet cells in the murine small intestinal crypt: experimental and modelling studies.
Authors:
Paulus, U; Loeffler, M; Zeidler, J; Owen, Gary; Potten, Christopher S
Abstract:
The objective of this study was to provide a new insight into the origin and lineage development of mucus-producing cells in the small intestinal crypt. For this, new experimental data were obtained from both crypt sections and whole mounts. Model simulation studies were undertaken to investigate which rules are most likely to govern the dynamic cellular development and goblet cell pedigree. We have measured the frequency of mucus-secreting goblet cells (using alcian blue and periodic acid Schiff's stains) at each cell position in the ileal murine crypt. These measurements, made on sections, overestimate the number of goblet cells because of the size and centripetal position of the stained cytoplasm. The correction factor for this overscoring has been measured to be 0.25 by two independent methods. The data suggest that there are about 12 functional goblet cells per crypt many of which retain an ability to divide. We have also determined the labelling index of the crypt goblet cells at each cell position. Spatially, goblet cells exhibit a small degree of clustering in the crypt and show a good mixture with columnar cells. We have adapted our earlier dynamic matrix-based computer stimulation model to take into account goblet cell differentiation. The modelling suggested the following conclusions: firstly, goblet cells do not have their own stem cells but share a common stem cell with the columnar cells; secondly, the goblet lineage differentiates from the transit population two to three generations before the end of the lineage; and thirdly, the decision to switch on goblet properties is stochastic at a specific step in the development of columnar cells.
Affiliation:
Department of Biometry, Medizinische Universitaetsklinik, Koeln, Germany.
Citation:
The differentiation and lineage development of goblet cells in the murine small intestinal crypt: experimental and modelling studies. 1993, 106 ( Pt 2):473-83 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
Oct-1993
URI:
http://hdl.handle.net/10541/101876
PubMed ID:
8282755
Type:
Article
Language:
en
ISSN:
0021-9533
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPaulus, Uen
dc.contributor.authorLoeffler, Men
dc.contributor.authorZeidler, Jen
dc.contributor.authorOwen, Garyen
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-06-07T17:04:47Z-
dc.date.available2010-06-07T17:04:47Z-
dc.date.issued1993-10-
dc.identifier.citationThe differentiation and lineage development of goblet cells in the murine small intestinal crypt: experimental and modelling studies. 1993, 106 ( Pt 2):473-83 J. Cell. Sci.en
dc.identifier.issn0021-9533-
dc.identifier.pmid8282755-
dc.identifier.urihttp://hdl.handle.net/10541/101876-
dc.description.abstractThe objective of this study was to provide a new insight into the origin and lineage development of mucus-producing cells in the small intestinal crypt. For this, new experimental data were obtained from both crypt sections and whole mounts. Model simulation studies were undertaken to investigate which rules are most likely to govern the dynamic cellular development and goblet cell pedigree. We have measured the frequency of mucus-secreting goblet cells (using alcian blue and periodic acid Schiff's stains) at each cell position in the ileal murine crypt. These measurements, made on sections, overestimate the number of goblet cells because of the size and centripetal position of the stained cytoplasm. The correction factor for this overscoring has been measured to be 0.25 by two independent methods. The data suggest that there are about 12 functional goblet cells per crypt many of which retain an ability to divide. We have also determined the labelling index of the crypt goblet cells at each cell position. Spatially, goblet cells exhibit a small degree of clustering in the crypt and show a good mixture with columnar cells. We have adapted our earlier dynamic matrix-based computer stimulation model to take into account goblet cell differentiation. The modelling suggested the following conclusions: firstly, goblet cells do not have their own stem cells but share a common stem cell with the columnar cells; secondly, the goblet lineage differentiates from the transit population two to three generations before the end of the lineage; and thirdly, the decision to switch on goblet properties is stochastic at a specific step in the development of columnar cells.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Division-
dc.subject.meshEpithelial Cells-
dc.subject.meshEpithelium-
dc.subject.meshIleum-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshModels, Biological-
dc.subject.meshMucus-
dc.subject.meshStem Cells-
dc.titleThe differentiation and lineage development of goblet cells in the murine small intestinal crypt: experimental and modelling studies.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biometry, Medizinische Universitaetsklinik, Koeln, Germany.en
dc.identifier.journalJournal of Cell Scienceen
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