2.50
Hdl Handle:
http://hdl.handle.net/10541/101875
Title:
The distribution of colony-forming cells in the kidney.
Authors:
Jen, Yee-Min; Hendry, Jolyon H
Abstract:
A method is described for producing outgrowths of small nephron segments (average 24 cells) in culture. The method was used to estimate an overall colony-forming efficiency of 4.6% for cells constituting the segments. Efficiency was found to be lower for thick segments (1%) than for thin segments (6%) from Henle's loop. The latter higher level indicates that precursor cells are concentrated near the middle of the nephron. For comparison, a two-dose irradiation technique was used to calculate a mean number of 5 +/- 2 (SE) clonogens per segment producing outgrowths. This tended to be higher than the value of about 1 calculated from the 65% of segments producing outgrowths, as expected if the remaining segments contained no clonogens.
Affiliation:
Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Citation:
The distribution of colony-forming cells in the kidney. 1993, 26 (3):263-9 Cell Prolif.
Journal:
Cell Proliferation
Issue Date:
May-1993
URI:
http://hdl.handle.net/10541/101875
DOI:
10.1111/j.1365-2184.1993.tb00024.x
PubMed ID:
8324073
Type:
Article
Language:
en
ISSN:
0960-7722
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorJen, Yee-Minen
dc.contributor.authorHendry, Jolyon Hen
dc.date.accessioned2010-06-07T17:00:32Z-
dc.date.available2010-06-07T17:00:32Z-
dc.date.issued1993-05-
dc.identifier.citationThe distribution of colony-forming cells in the kidney. 1993, 26 (3):263-9 Cell Prolif.en
dc.identifier.issn0960-7722-
dc.identifier.pmid8324073-
dc.identifier.doi10.1111/j.1365-2184.1993.tb00024.x-
dc.identifier.urihttp://hdl.handle.net/10541/101875-
dc.description.abstractA method is described for producing outgrowths of small nephron segments (average 24 cells) in culture. The method was used to estimate an overall colony-forming efficiency of 4.6% for cells constituting the segments. Efficiency was found to be lower for thick segments (1%) than for thin segments (6%) from Henle's loop. The latter higher level indicates that precursor cells are concentrated near the middle of the nephron. For comparison, a two-dose irradiation technique was used to calculate a mean number of 5 +/- 2 (SE) clonogens per segment producing outgrowths. This tended to be higher than the value of about 1 calculated from the 65% of segments producing outgrowths, as expected if the remaining segments contained no clonogens.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshKidney Glomerulus-
dc.subject.meshKidney Tubules-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshOrgan Culture Techniques-
dc.subject.meshStem Cells-
dc.titleThe distribution of colony-forming cells in the kidney.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.en
dc.identifier.journalCell Proliferationen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.