Stimulator of proliferation of spleen colony-forming cells in acute sterile inflammation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/101873
Title:
Stimulator of proliferation of spleen colony-forming cells in acute sterile inflammation.
Authors:
Milenković, P; Ivanović, Z; Lukić, M L; Kataranovski, M; Lord, Brian I
Abstract:
The presence and activity of a spleen colony-forming cell (CFU-S) proliferation stimulator was investigated in rat bone marrow after induction of sterile inflammation. Wistar rats were treated intraperitoneally with two 15 ml injections of 3.5% polyvinylpyrrolidone (PVP) at 18 h intervals, and the presence of CFU-S proliferation stimulator determined in bone marrow 6, 20 and 24 h after the first injection. The marrow of these mice was used to condition medium which was then fractionated using Amicon Diaflo ultrafiltration membranes. The 30-50 kDa fraction, taken from 20h post-PVP-bone marrow extract, was found to induce cycling of d8-CFU-S in normal mouse bone marrow. This activity was not related to the presence of interleukins-1, -2 or -6 like activities in the material tested. The results demonstrate the existence of CFU-S proliferation stimulator in the bone marrow of rats with sterile inflammation (i.e. in an in vivo tissue response to non-specific cell stimulation), similar to that originally described as a macrophage product in mouse bone marrow after treatment with a variety of cytotoxic agents.
Affiliation:
Institute for Medical Research, University of Beograd, Yugoslavia (Serbia).
Citation:
Stimulator of proliferation of spleen colony-forming cells in acute sterile inflammation. 1993, 26 (6):503-10 Cell Prolif.
Journal:
Cell Proliferation
Issue Date:
Nov-1993
URI:
http://hdl.handle.net/10541/101873
DOI:
10.1111/j.1365-2184.1993.tb00028.x
PubMed ID:
9116117
Type:
Article
Language:
en
ISSN:
0960-7722
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMilenković, Pen
dc.contributor.authorIvanović, Zen
dc.contributor.authorLukić, M Len
dc.contributor.authorKataranovski, Men
dc.contributor.authorLord, Brian Ien
dc.date.accessioned2010-06-07T16:54:19Z-
dc.date.available2010-06-07T16:54:19Z-
dc.date.issued1993-11-
dc.identifier.citationStimulator of proliferation of spleen colony-forming cells in acute sterile inflammation. 1993, 26 (6):503-10 Cell Prolif.en
dc.identifier.issn0960-7722-
dc.identifier.pmid9116117-
dc.identifier.doi10.1111/j.1365-2184.1993.tb00028.x-
dc.identifier.urihttp://hdl.handle.net/10541/101873-
dc.description.abstractThe presence and activity of a spleen colony-forming cell (CFU-S) proliferation stimulator was investigated in rat bone marrow after induction of sterile inflammation. Wistar rats were treated intraperitoneally with two 15 ml injections of 3.5% polyvinylpyrrolidone (PVP) at 18 h intervals, and the presence of CFU-S proliferation stimulator determined in bone marrow 6, 20 and 24 h after the first injection. The marrow of these mice was used to condition medium which was then fractionated using Amicon Diaflo ultrafiltration membranes. The 30-50 kDa fraction, taken from 20h post-PVP-bone marrow extract, was found to induce cycling of d8-CFU-S in normal mouse bone marrow. This activity was not related to the presence of interleukins-1, -2 or -6 like activities in the material tested. The results demonstrate the existence of CFU-S proliferation stimulator in the bone marrow of rats with sterile inflammation (i.e. in an in vivo tissue response to non-specific cell stimulation), similar to that originally described as a macrophage product in mouse bone marrow after treatment with a variety of cytotoxic agents.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshBone Marrow-
dc.subject.meshCulture Media, Conditioned-
dc.subject.meshGrowth Substances-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshInflammation-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshPovidone-
dc.subject.meshRats-
dc.subject.meshRats, Wistar-
dc.subject.meshSpleen-
dc.titleStimulator of proliferation of spleen colony-forming cells in acute sterile inflammation.en
dc.typeArticleen
dc.contributor.departmentInstitute for Medical Research, University of Beograd, Yugoslavia (Serbia).en
dc.identifier.journalCell Proliferationen

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