Somatic mutation, monoclonality and stochastic models of stem cell organization in the intestinal crypt.

2.50
Hdl Handle:
http://hdl.handle.net/10541/101862
Title:
Somatic mutation, monoclonality and stochastic models of stem cell organization in the intestinal crypt.
Authors:
Loeffler, M; Birke, A; Winton, D; Potten, Christopher S
Abstract:
Among highly proliferating tissues the intestinal tissue is of particular interest. Techniques are available that permit an insight into how intestinal crypts as the basic macroscopic tissue unit are regenerated from a small population of self-maintaining stem cells. However, neither the precise number of these stem cells nor their properties are known. We have recently suggested a model of stem cell organization which explains the life cycle of murine intestinal crypts, their birth (by crypt fission) and extinction rates, as well as their size distribution on a quantitative basis (Loeffler & Grossman, 1991). The model assumptions involve two stochastic branching processes, one for the growth of several independent indistinguishable stem cells and a second for a threshold dependent crypt fission process. New data have now become available challenging the above concept. They relate to the conversion of crypts to monoclonal phenotypic expression after mutagenic events, presumably taking place in single stem cells. A detailed analysis of these data is shown here utilizing a more elaborate version of the above model. The new data are consistent with this model within the range of parameters predicted previously. We conclude that the cellular regeneration of intestinal crypts can be explained on the basis of several indistinguishable stem cells which can replace each other.
Affiliation:
Department of Biometry, Universität zu Koeln, Germany.
Citation:
Somatic mutation, monoclonality and stochastic models of stem cell organization in the intestinal crypt. 1993, 160 (4):471-91 J. Theor. Biol.
Journal:
Journal of Theoretical Biology
Issue Date:
21-Feb-1993
URI:
http://hdl.handle.net/10541/101862
DOI:
10.1006/jtbi.1993.1031
PubMed ID:
8501919
Type:
Article
Language:
en
ISSN:
0022-5193
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLoeffler, Men
dc.contributor.authorBirke, Aen
dc.contributor.authorWinton, Den
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-06-07T16:55:15Z-
dc.date.available2010-06-07T16:55:15Z-
dc.date.issued1993-02-21-
dc.identifier.citationSomatic mutation, monoclonality and stochastic models of stem cell organization in the intestinal crypt. 1993, 160 (4):471-91 J. Theor. Biol.en
dc.identifier.issn0022-5193-
dc.identifier.pmid8501919-
dc.identifier.doi10.1006/jtbi.1993.1031-
dc.identifier.urihttp://hdl.handle.net/10541/101862-
dc.description.abstractAmong highly proliferating tissues the intestinal tissue is of particular interest. Techniques are available that permit an insight into how intestinal crypts as the basic macroscopic tissue unit are regenerated from a small population of self-maintaining stem cells. However, neither the precise number of these stem cells nor their properties are known. We have recently suggested a model of stem cell organization which explains the life cycle of murine intestinal crypts, their birth (by crypt fission) and extinction rates, as well as their size distribution on a quantitative basis (Loeffler & Grossman, 1991). The model assumptions involve two stochastic branching processes, one for the growth of several independent indistinguishable stem cells and a second for a threshold dependent crypt fission process. New data have now become available challenging the above concept. They relate to the conversion of crypts to monoclonal phenotypic expression after mutagenic events, presumably taking place in single stem cells. A detailed analysis of these data is shown here utilizing a more elaborate version of the above model. The new data are consistent with this model within the range of parameters predicted previously. We conclude that the cellular regeneration of intestinal crypts can be explained on the basis of several indistinguishable stem cells which can replace each other.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Cycle-
dc.subject.meshEpithelial Cells-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshMice-
dc.subject.meshModels, Biological-
dc.subject.meshMutation-
dc.subject.meshStem Cells-
dc.subject.meshStochastic Processes-
dc.titleSomatic mutation, monoclonality and stochastic models of stem cell organization in the intestinal crypt.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biometry, Universität zu Koeln, Germany.en
dc.identifier.journalJournal of Theoretical Biologyen
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