The one-electron reduction potential of several substrates can be related to their reduction rates by cytochrome P-450 reductase.

2.50
Hdl Handle:
http://hdl.handle.net/10541/101861
Title:
The one-electron reduction potential of several substrates can be related to their reduction rates by cytochrome P-450 reductase.
Authors:
Butler, John; Hoey, Brigid M
Abstract:
The rates of reduction of 27 compounds by purified cytochrome P-450 reductase have been studied and correlated with the one-electron reduction potentials E7(X/X.-). For compounds with reduction potentials between approx. -400 and -165 mV, there was a good correlation between the rates and E7(X/X.-); log rate (mumol/min per mg) = (2.38 +/- 0.08) + (0.0061 +/- 0.0003)E7 (mV). Compounds with potentials more positive than -165 mV were shown to deviate from the correlation. It is shown that under the standard conditions of the assay, these compounds undergo two-electron reduction by NADPH rather than one-electron reduction with the enzyme. As a consequence of the study, the rate constants for the reaction of superoxide radicals with native and acetylated cytochrome c were also determined to be (4.5 +/- 0.5).10(5) and (1.5 +/- 0.15).10(5) M-1 s-1, respectively.
Affiliation:
CRC Department of Biophysical Chemistry, Paterson Institute for Cancer Research, Christie Hospital, NHS Trust, Manchester, UK.
Citation:
The one-electron reduction potential of several substrates can be related to their reduction rates by cytochrome P-450 reductase. 1993, 1161 (1):73-8 Biochim. Biophys. Acta
Journal:
Biochimica et Biophysica Acta
Issue Date:
15-Jan-1993
URI:
http://hdl.handle.net/10541/101861
PubMed ID:
8380722
Type:
Article
Language:
en
ISSN:
0006-3002
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorButler, Johnen
dc.contributor.authorHoey, Brigid Men
dc.date.accessioned2010-06-07T16:11:13Z-
dc.date.available2010-06-07T16:11:13Z-
dc.date.issued1993-01-15-
dc.identifier.citationThe one-electron reduction potential of several substrates can be related to their reduction rates by cytochrome P-450 reductase. 1993, 1161 (1):73-8 Biochim. Biophys. Actaen
dc.identifier.issn0006-3002-
dc.identifier.pmid8380722-
dc.identifier.urihttp://hdl.handle.net/10541/101861-
dc.description.abstractThe rates of reduction of 27 compounds by purified cytochrome P-450 reductase have been studied and correlated with the one-electron reduction potentials E7(X/X.-). For compounds with reduction potentials between approx. -400 and -165 mV, there was a good correlation between the rates and E7(X/X.-); log rate (mumol/min per mg) = (2.38 +/- 0.08) + (0.0061 +/- 0.0003)E7 (mV). Compounds with potentials more positive than -165 mV were shown to deviate from the correlation. It is shown that under the standard conditions of the assay, these compounds undergo two-electron reduction by NADPH rather than one-electron reduction with the enzyme. As a consequence of the study, the rate constants for the reaction of superoxide radicals with native and acetylated cytochrome c were also determined to be (4.5 +/- 0.5).10(5) and (1.5 +/- 0.15).10(5) M-1 s-1, respectively.en
dc.language.isoenen
dc.subject.meshCytochrome c Group-
dc.subject.meshElectrons-
dc.subject.meshKinetics-
dc.subject.meshNADH, NADPH Oxidoreductases-
dc.subject.meshOxidation-Reduction-
dc.subject.meshSuperoxides-
dc.titleThe one-electron reduction potential of several substrates can be related to their reduction rates by cytochrome P-450 reductase.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Biophysical Chemistry, Paterson Institute for Cancer Research, Christie Hospital, NHS Trust, Manchester, UK.en
dc.identifier.journalBiochimica et Biophysica Actaen
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