The role of metallothionein, glutathione, glutathione S-transferases and DNA repair in resistance to platinum drugs in a series of L1210 cell lines made resistant to anticancer platinum agents.

2.50
Hdl Handle:
http://hdl.handle.net/10541/101832
Title:
The role of metallothionein, glutathione, glutathione S-transferases and DNA repair in resistance to platinum drugs in a series of L1210 cell lines made resistant to anticancer platinum agents.
Authors:
Hrubisko, M; McGown, Alan T; Fox, Brian W
Abstract:
The glutathione contents, glutathione S-transferase activities and metallothionein contents have been measured in a series of L1210 cell lines which show decreased sensitivities to platinum drugs. Resistance to cisplatinum cisDDP, cis-diamminedichloroplatinum (II)] and chip [ioproplatin, cisdichloro-bis-isopropylamine-trans dihydroxy platinum IV] was found to correlate with glutathione levels but not metallothionein. Conversely, resistance to tetraplatin was found to be correlated with metallothionein but not glutathione levels. However, depletion of glutathione by buthionine 1-sulphoximine sensitizes all cell lines to the effects of cisDDP, chip and tetraplatin [d,1-trans-tetrachloro-1,2-diamino-cyclohexanplatinum (IV)]. Inhibition of DNA repair by aphidicholin or caffeine also partially restored sensitivity to these platinum drugs. These results indicate the complexity of the changes occurring upon the development of drug resistance.
Affiliation:
CRC Dept of Experimental Chemotherapy, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.
Citation:
The role of metallothionein, glutathione, glutathione S-transferases and DNA repair in resistance to platinum drugs in a series of L1210 cell lines made resistant to anticancer platinum agents. 1993, 45 (1):253-6 Biochem. Pharmacol.
Journal:
Biochemical Pharmacology
Issue Date:
7-Jan-1993
URI:
http://hdl.handle.net/10541/101832
DOI:
10.1016/0006-2952(93)90399-H
PubMed ID:
8424817
Type:
Article
Language:
en
ISSN:
0006-2952
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHrubisko, Men
dc.contributor.authorMcGown, Alan Ten
dc.contributor.authorFox, Brian Wen
dc.date.accessioned2010-06-07T16:10:32Z-
dc.date.available2010-06-07T16:10:32Z-
dc.date.issued1993-01-07-
dc.identifier.citationThe role of metallothionein, glutathione, glutathione S-transferases and DNA repair in resistance to platinum drugs in a series of L1210 cell lines made resistant to anticancer platinum agents. 1993, 45 (1):253-6 Biochem. Pharmacol.en
dc.identifier.issn0006-2952-
dc.identifier.pmid8424817-
dc.identifier.doi10.1016/0006-2952(93)90399-H-
dc.identifier.urihttp://hdl.handle.net/10541/101832-
dc.description.abstractThe glutathione contents, glutathione S-transferase activities and metallothionein contents have been measured in a series of L1210 cell lines which show decreased sensitivities to platinum drugs. Resistance to cisplatinum cisDDP, cis-diamminedichloroplatinum (II)] and chip [ioproplatin, cisdichloro-bis-isopropylamine-trans dihydroxy platinum IV] was found to correlate with glutathione levels but not metallothionein. Conversely, resistance to tetraplatin was found to be correlated with metallothionein but not glutathione levels. However, depletion of glutathione by buthionine 1-sulphoximine sensitizes all cell lines to the effects of cisDDP, chip and tetraplatin [d,1-trans-tetrachloro-1,2-diamino-cyclohexanplatinum (IV)]. Inhibition of DNA repair by aphidicholin or caffeine also partially restored sensitivity to these platinum drugs. These results indicate the complexity of the changes occurring upon the development of drug resistance.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subjectLeukaemiaen
dc.subject.meshAnimals-
dc.subject.meshCaffeine-
dc.subject.meshCisplatin-
dc.subject.meshDNA Repair-
dc.subject.meshDrug Resistance-
dc.subject.meshGlutathione-
dc.subject.meshGlutathione Transferase-
dc.subject.meshLeukemia L1210-
dc.subject.meshMetallothionein-
dc.subject.meshOrganoplatinum Compounds-
dc.subject.meshTumor Cells, Cultured-
dc.titleThe role of metallothionein, glutathione, glutathione S-transferases and DNA repair in resistance to platinum drugs in a series of L1210 cell lines made resistant to anticancer platinum agents.en
dc.typeArticleen
dc.contributor.departmentCRC Dept of Experimental Chemotherapy, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.en
dc.identifier.journalBiochemical Pharmacologyen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.