Multiple signaling pathways mediate anti-Ig and IL-4-induced early response gene expression in human tonsillar B cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/100406
Title:
Multiple signaling pathways mediate anti-Ig and IL-4-induced early response gene expression in human tonsillar B cells.
Authors:
Murphy, J J; Norton, John D
Abstract:
We have analyzed the relationship between the signaling pathways coupled to surface immunoglobulin and interleukin (IL)-4 receptors in human B cells from the patterns of expression of a panel of phorbol ester-inducible early response genes (ERG) activated by anti-IgM and IL-4 stimulation in vitro. Anti-IgM stimulation led to the induction of all eleven ERG tested. Two of these, the proto-oncogene, c-fos and an anonymous ERG 1R20 were insensitive to protein kinase C (PKC) inhibition with the drug, staurosporine and retained inducibility after down-regulation of PKC activity by purging with phorbol ester. These observations are consistent with previous data showing anti-IgM signaling through both PKC-dependent and PKC-independent pathways. c-fos and 1R20 were also the only ERG inducible in response to IL-4 stimulation and whilst ionomycin induced only c-fos, dibutyryl cyclic adenosine monophosphate stimulation led to induction of both c-fos and 1R20. These observations lend support to a role for the adenylate cyclase pathway being important for coupling of IL-4-generated signals to B cells responses. None of the anti-IgM-responsive ERG was further induced when B cells were co-stimulated with a combination of anti-IgM and IL-4, suggesting that the signaling cascades from these two agents are integrated downstream of third messenger pathways to synergistically promote B cell proliferation.
Affiliation:
Division of Life Sciences, King's College London, GB.
Citation:
Multiple signaling pathways mediate anti-Ig and IL-4-induced early response gene expression in human tonsillar B cells. 1993, 23 (11):2876-81 Eur. J. Immunol.
Journal:
European Journal of Immunology
Issue Date:
Nov-1993
URI:
http://hdl.handle.net/10541/100406
DOI:
10.1002/eji.1830231122
PubMed ID:
7693480
Type:
Article
Language:
en
ISSN:
0014-2980
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMurphy, J Jen
dc.contributor.authorNorton, John Den
dc.date.accessioned2010-06-07T11:07:31Z-
dc.date.available2010-06-07T11:07:31Z-
dc.date.issued1993-11-
dc.identifier.citationMultiple signaling pathways mediate anti-Ig and IL-4-induced early response gene expression in human tonsillar B cells. 1993, 23 (11):2876-81 Eur. J. Immunol.en
dc.identifier.issn0014-2980-
dc.identifier.pmid7693480-
dc.identifier.doi10.1002/eji.1830231122-
dc.identifier.urihttp://hdl.handle.net/10541/100406-
dc.description.abstractWe have analyzed the relationship between the signaling pathways coupled to surface immunoglobulin and interleukin (IL)-4 receptors in human B cells from the patterns of expression of a panel of phorbol ester-inducible early response genes (ERG) activated by anti-IgM and IL-4 stimulation in vitro. Anti-IgM stimulation led to the induction of all eleven ERG tested. Two of these, the proto-oncogene, c-fos and an anonymous ERG 1R20 were insensitive to protein kinase C (PKC) inhibition with the drug, staurosporine and retained inducibility after down-regulation of PKC activity by purging with phorbol ester. These observations are consistent with previous data showing anti-IgM signaling through both PKC-dependent and PKC-independent pathways. c-fos and 1R20 were also the only ERG inducible in response to IL-4 stimulation and whilst ionomycin induced only c-fos, dibutyryl cyclic adenosine monophosphate stimulation led to induction of both c-fos and 1R20. These observations lend support to a role for the adenylate cyclase pathway being important for coupling of IL-4-generated signals to B cells responses. None of the anti-IgM-responsive ERG was further induced when B cells were co-stimulated with a combination of anti-IgM and IL-4, suggesting that the signaling cascades from these two agents are integrated downstream of third messenger pathways to synergistically promote B cell proliferation.en
dc.language.isoenen
dc.subject.meshAlkaloids-
dc.subject.meshAntibodies, Anti-Idiotypic-
dc.subject.meshB-Lymphocytes-
dc.subject.meshGene Expression-
dc.subject.meshGenes, MHC Class II-
dc.subject.meshHumans-
dc.subject.meshImmunoglobulin M-
dc.subject.meshInterleukin-4-
dc.subject.meshLymphocyte Activation-
dc.subject.meshProtein Kinase C-
dc.subject.meshRNA-
dc.subject.meshSignal Transduction-
dc.subject.meshStaurosporine-
dc.titleMultiple signaling pathways mediate anti-Ig and IL-4-induced early response gene expression in human tonsillar B cells.en
dc.typeArticleen
dc.contributor.departmentDivision of Life Sciences, King's College London, GB.en
dc.identifier.journalEuropean Journal of Immunologyen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.