The expression of matrix metalloproteinases and their inhibitors by pig synovial cells and their regulation by combinations of cytokines and growth factors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/100400
Title:
The expression of matrix metalloproteinases and their inhibitors by pig synovial cells and their regulation by combinations of cytokines and growth factors.
Authors:
Legendre, P; Richards, C D; Rafferty, Joseph A; Dew, G W; Reynolds, J J
Abstract:
1. Pig synovial fibroblasts in culture were studied to determine if they were an easily reproducible model system for studying the actions of cytokines and growth factors on human synovial cells. The biochemical analyses were conducted by activity assays, enzymography and Northern blot. 2. Human recombinant interleukin-1 alpha, basic fibroblast growth factor and transforming growth factor-beta 1 were studied in combinations because of their known involvement in controlling tissue remodelling. 3. The response of pig fibroblasts to these agents, in terms of the expression of matrix metalloproteinases (collagenase, gelatinase and stromelysin) and their inhibitors (TIMPs), show that they behave similarly enough to human cells for use when supplies of human primary cells are unavailable.
Affiliation:
Cell and Molecular Biology Department, Strangeways Research Laboratory, Cambridge, U.K.
Citation:
The expression of matrix metalloproteinases and their inhibitors by pig synovial cells and their regulation by combinations of cytokines and growth factors. 1993, 106 (3):691-704 Comp. Biochem. Physiol., B
Journal:
Comparative Biochemistry and Physiology. B, Comparative Biochemistry
Issue Date:
Nov-1993
URI:
http://hdl.handle.net/10541/100400
DOI:
10.1016/0305-0491(93)90151-T
PubMed ID:
8281764
Type:
Article
Language:
en
ISSN:
0305-0491
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLegendre, Pen
dc.contributor.authorRichards, C Den
dc.contributor.authorRafferty, Joseph Aen
dc.contributor.authorDew, G Wen
dc.contributor.authorReynolds, J Jen
dc.date.accessioned2010-06-07T10:47:53Z-
dc.date.available2010-06-07T10:47:53Z-
dc.date.issued1993-11-
dc.identifier.citationThe expression of matrix metalloproteinases and their inhibitors by pig synovial cells and their regulation by combinations of cytokines and growth factors. 1993, 106 (3):691-704 Comp. Biochem. Physiol., Ben
dc.identifier.issn0305-0491-
dc.identifier.pmid8281764-
dc.identifier.doi10.1016/0305-0491(93)90151-T-
dc.identifier.urihttp://hdl.handle.net/10541/100400-
dc.description.abstract1. Pig synovial fibroblasts in culture were studied to determine if they were an easily reproducible model system for studying the actions of cytokines and growth factors on human synovial cells. The biochemical analyses were conducted by activity assays, enzymography and Northern blot. 2. Human recombinant interleukin-1 alpha, basic fibroblast growth factor and transforming growth factor-beta 1 were studied in combinations because of their known involvement in controlling tissue remodelling. 3. The response of pig fibroblasts to these agents, in terms of the expression of matrix metalloproteinases (collagenase, gelatinase and stromelysin) and their inhibitors (TIMPs), show that they behave similarly enough to human cells for use when supplies of human primary cells are unavailable.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCells, Cultured-
dc.subject.meshCytokines-
dc.subject.meshExtracellular Matrix-
dc.subject.meshFibroblasts-
dc.subject.meshGrowth Substances-
dc.subject.meshHumans-
dc.subject.meshMetalloendopeptidases-
dc.subject.meshSwine-
dc.subject.meshSynovial Membrane-
dc.subject.meshTranscription, Genetic-
dc.titleThe expression of matrix metalloproteinases and their inhibitors by pig synovial cells and their regulation by combinations of cytokines and growth factors.en
dc.typeArticleen
dc.contributor.departmentCell and Molecular Biology Department, Strangeways Research Laboratory, Cambridge, U.K.en
dc.identifier.journalComparative Biochemistry and Physiology. B, Comparative Biochemistryen
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