Early response gene signalling cascades activated by ionising radiation in primary human B cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/100398
Title:
Early response gene signalling cascades activated by ionising radiation in primary human B cells.
Authors:
Wilson, R E; Taylor, S L; Atherton, Graham T; Johnston, D; Waters, C M; Norton, John D
Abstract:
We have used a panel of 13 protein kinase C-responsive immediate early gene probes to dissect the cellular signalling pathways activated by ionising gamma radiation in primary human B cells. Of these 13 genes, a delayed transient induction was observed for only 8: c-fos, c-jun, jun-B, jun-D, c-myc, ergI/krox 24 and two 'anonymous' genes, 3L3 and 19A. Expression of c-myc and c-fos mRNAs was paralleled by the appearance of their encoded proteins suggesting that these oncoproteins may couple radiation signalling to cellular responses. Of three protein kinase C-coupled transcription factors examined by gel retardation assay, (AP1, NF kappa B, EgrK/Krox24) only NF kappa B and, to a lesser extent, AP1 was stimulated in response to irradiation. These observations are not obviously compatible with a simple model invoking protein kinase C in radiation signalling in primary B cells and suggest that the pleiotropic effects of ionising radiation on this cell type are mediated through a distinct cellular signalling cascade.
Affiliation:
CRC Department of Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
Early response gene signalling cascades activated by ionising radiation in primary human B cells. 1993, 8 (12):3229-37 Oncogene
Journal:
Oncogene
Issue Date:
Dec-1993
URI:
http://hdl.handle.net/10541/100398
PubMed ID:
8247526
Type:
Article
Language:
en
ISSN:
0950-9232
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWilson, R Een
dc.contributor.authorTaylor, S Len
dc.contributor.authorAtherton, Graham Ten
dc.contributor.authorJohnston, Den
dc.contributor.authorWaters, C Men
dc.contributor.authorNorton, John Den
dc.date.accessioned2010-06-07T10:36:22Z-
dc.date.available2010-06-07T10:36:22Z-
dc.date.issued1993-12-
dc.identifier.citationEarly response gene signalling cascades activated by ionising radiation in primary human B cells. 1993, 8 (12):3229-37 Oncogeneen
dc.identifier.issn0950-9232-
dc.identifier.pmid8247526-
dc.identifier.urihttp://hdl.handle.net/10541/100398-
dc.description.abstractWe have used a panel of 13 protein kinase C-responsive immediate early gene probes to dissect the cellular signalling pathways activated by ionising gamma radiation in primary human B cells. Of these 13 genes, a delayed transient induction was observed for only 8: c-fos, c-jun, jun-B, jun-D, c-myc, ergI/krox 24 and two 'anonymous' genes, 3L3 and 19A. Expression of c-myc and c-fos mRNAs was paralleled by the appearance of their encoded proteins suggesting that these oncoproteins may couple radiation signalling to cellular responses. Of three protein kinase C-coupled transcription factors examined by gel retardation assay, (AP1, NF kappa B, EgrK/Krox24) only NF kappa B and, to a lesser extent, AP1 was stimulated in response to irradiation. These observations are not obviously compatible with a simple model invoking protein kinase C in radiation signalling in primary B cells and suggest that the pleiotropic effects of ionising radiation on this cell type are mediated through a distinct cellular signalling cascade.en
dc.language.isoenen
dc.subject.meshB-Lymphocytes-
dc.subject.meshBase Sequence-
dc.subject.meshBlotting, Northern-
dc.subject.meshBlotting, Western-
dc.subject.meshCells, Cultured-
dc.subject.meshDNA-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshEarly Growth Response Protein 1-
dc.subject.meshGene Expression Regulation-
dc.subject.meshGenes, Immediate-Early-
dc.subject.meshHumans-
dc.subject.meshImmediate-Early Proteins-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshNF-kappa B-
dc.subject.meshProtein Kinase C-
dc.subject.meshProto-Oncogene Proteins c-fos-
dc.subject.meshProto-Oncogene Proteins c-jun-
dc.subject.meshProto-Oncogene Proteins c-myc-
dc.subject.meshRNA, Messenger-
dc.subject.meshRadiation, Ionizing-
dc.subject.meshRetroviridae Proteins, Oncogenic-
dc.subject.meshSignal Transduction-
dc.subject.meshTetradecanoylphorbol Acetate-
dc.subject.meshTranscription Factors-
dc.subject.meshTranscription, Genetic-
dc.titleEarly response gene signalling cascades activated by ionising radiation in primary human B cells.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalOncogeneen

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