Suppression of apoptosis allows differentiation and development of a multipotent hemopoietic cell line in the absence of added growth factors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/100378
Title:
Suppression of apoptosis allows differentiation and development of a multipotent hemopoietic cell line in the absence of added growth factors.
Authors:
Fairbairn, Leslie J; Cowling, Graham J; Reipert, B M; Dexter, T Michael
Abstract:
In the absence of growth factors, hemopoietic cells die rapidly by the process of apoptosis. Transfection of the human bcl-2 gene into an interleukin-3 (IL-3)-dependent, multipotent hemopoietic cell line allowed these cells to survive in the absence of IL-3, both in serum-containing and serum-deprived conditions, and this survival was accompanied by multilineage differentiation. Moreover, single cell experiments showed that differentiation could occur in the absence of cell division. While these data do not rule out the possibility that growth factors can influence the lineage choice of multipotent cells, they suggest that exposure to growth factors may not be obligatory for the differentiation of stem cells. The data also support the hypothesis that differentiation is intrinsically determined and that the role of the hemopoietic growth factors is enabling rather than inductive.
Affiliation:
Cancer Research Campaign Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, England.
Citation:
Suppression of apoptosis allows differentiation and development of a multipotent hemopoietic cell line in the absence of added growth factors. 1993, 74 (5):823-32 Cell
Journal:
Cell
Issue Date:
10-Sep-1993
URI:
http://hdl.handle.net/10541/100378
DOI:
10.1016/0092-8674(93)90462-Y
PubMed ID:
7690686
Type:
Article
Language:
en
ISSN:
0092-8674
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorFairbairn, Leslie Jen
dc.contributor.authorCowling, Graham Jen
dc.contributor.authorReipert, B Men
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-06-07T10:22:11Z-
dc.date.available2010-06-07T10:22:11Z-
dc.date.issued1993-09-10-
dc.identifier.citationSuppression of apoptosis allows differentiation and development of a multipotent hemopoietic cell line in the absence of added growth factors. 1993, 74 (5):823-32 Cellen
dc.identifier.issn0092-8674-
dc.identifier.pmid7690686-
dc.identifier.doi10.1016/0092-8674(93)90462-Y-
dc.identifier.urihttp://hdl.handle.net/10541/100378-
dc.description.abstractIn the absence of growth factors, hemopoietic cells die rapidly by the process of apoptosis. Transfection of the human bcl-2 gene into an interleukin-3 (IL-3)-dependent, multipotent hemopoietic cell line allowed these cells to survive in the absence of IL-3, both in serum-containing and serum-deprived conditions, and this survival was accompanied by multilineage differentiation. Moreover, single cell experiments showed that differentiation could occur in the absence of cell division. While these data do not rule out the possibility that growth factors can influence the lineage choice of multipotent cells, they suggest that exposure to growth factors may not be obligatory for the differentiation of stem cells. The data also support the hypothesis that differentiation is intrinsically determined and that the role of the hemopoietic growth factors is enabling rather than inductive.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshBlotting, Northern-
dc.subject.meshBlotting, Southern-
dc.subject.meshCell Line-
dc.subject.meshCell Survival-
dc.subject.meshCulture Media-
dc.subject.meshCulture Media, Serum-Free-
dc.subject.meshDNA-
dc.subject.meshFlow Cytometry-
dc.subject.meshGTP-Binding Proteins-
dc.subject.meshHumans-
dc.subject.meshInterleukin-3-
dc.subject.meshMice-
dc.subject.meshProto-Oncogene Proteins-
dc.subject.meshProto-Oncogene Proteins c-bcl-2-
dc.subject.meshProto-Oncogenes-
dc.subject.meshRNA-
dc.subject.meshStem Cells-
dc.subject.meshTransfection-
dc.titleSuppression of apoptosis allows differentiation and development of a multipotent hemopoietic cell line in the absence of added growth factors.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, England.en
dc.identifier.journalCellen

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