A relationship between residual stromal damage in hematopoietic tissue and the functional activity of granulocytes.

2.50
Hdl Handle:
http://hdl.handle.net/10541/100335
Title:
A relationship between residual stromal damage in hematopoietic tissue and the functional activity of granulocytes.
Authors:
Gaitán, S; Tejero, C; Humphreys, E R; Lord, Brian I
Abstract:
This paper analyzes the function of mouse granulocytes in the long term, after external irradiation with x- and gamma-rays and 239Pu contamination at different gestational ages and in a variety of culture conditions. These treatments can produce persistent defects in the stroma, which regulates hematopoiesis. Superoxide-anion production has been measured in granulocytes from peripheral blood and from long-term bone marrow cultures (LTBMC). A significant enhancement of O2- is produced using single or fractionated doses of x-rays; however, little or no increase is observed with gamma-rays. With 239Pu, enhancement of O2- depends on gestational age at contamination. The absence of hydrocortisone (HC) in LTBMC and the irradiation of the adherent layer with 15 Gy stimulate O2- production. The increased production of O2- appears to be correlated with an excess of colony-stimulating factors (CSFs) released to the supernatant by stromal cells. Neutralization with anti-granulocyte-macrophage CSF (anti-GM-CSF) monoclonal antibody shows that GM-CSF is the main factor produced. In summary, conditions that lead to residual stromal damage also result in the generation of granulocytes that are functionally primed for excess superoxide-anion production.
Affiliation:
Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Citation:
A relationship between residual stromal damage in hematopoietic tissue and the functional activity of granulocytes. 1993, 21 (9):1227-32 Exp. Hematol.
Journal:
Experimental Hematology
Issue Date:
Aug-1993
URI:
http://hdl.handle.net/10541/100335
PubMed ID:
7687218
Type:
Article
Language:
en
ISSN:
0301-472X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGaitán, Sen
dc.contributor.authorTejero, Cen
dc.contributor.authorHumphreys, E Ren
dc.contributor.authorLord, Brian Ien
dc.date.accessioned2010-06-05T10:50:18Z-
dc.date.available2010-06-05T10:50:18Z-
dc.date.issued1993-08-
dc.identifier.citationA relationship between residual stromal damage in hematopoietic tissue and the functional activity of granulocytes. 1993, 21 (9):1227-32 Exp. Hematol.en
dc.identifier.issn0301-472X-
dc.identifier.pmid7687218-
dc.identifier.urihttp://hdl.handle.net/10541/100335-
dc.description.abstractThis paper analyzes the function of mouse granulocytes in the long term, after external irradiation with x- and gamma-rays and 239Pu contamination at different gestational ages and in a variety of culture conditions. These treatments can produce persistent defects in the stroma, which regulates hematopoiesis. Superoxide-anion production has been measured in granulocytes from peripheral blood and from long-term bone marrow cultures (LTBMC). A significant enhancement of O2- is produced using single or fractionated doses of x-rays; however, little or no increase is observed with gamma-rays. With 239Pu, enhancement of O2- depends on gestational age at contamination. The absence of hydrocortisone (HC) in LTBMC and the irradiation of the adherent layer with 15 Gy stimulate O2- production. The increased production of O2- appears to be correlated with an excess of colony-stimulating factors (CSFs) released to the supernatant by stromal cells. Neutralization with anti-granulocyte-macrophage CSF (anti-GM-CSF) monoclonal antibody shows that GM-CSF is the main factor produced. In summary, conditions that lead to residual stromal damage also result in the generation of granulocytes that are functionally primed for excess superoxide-anion production.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBone Marrow Cells-
dc.subject.meshCells, Cultured-
dc.subject.meshFemale-
dc.subject.meshGranulocyte Colony-Stimulating Factor-
dc.subject.meshGranulocytes-
dc.subject.meshMacrophages-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshPlutonium-
dc.subject.meshRecombinant Proteins-
dc.subject.meshStem Cells-
dc.subject.meshSuperoxides-
dc.subject.meshWhole-Body Irradiation-
dc.titleA relationship between residual stromal damage in hematopoietic tissue and the functional activity of granulocytes.en
dc.typeArticleen
dc.contributor.departmentDepartamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.en
dc.identifier.journalExperimental Hematologyen
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