An immediate early human gene encodes an Id-like helix-loop-helix protein and is regulated by protein kinase C activation in diverse cell types.

2.50
Hdl Handle:
http://hdl.handle.net/10541/100233
Title:
An immediate early human gene encodes an Id-like helix-loop-helix protein and is regulated by protein kinase C activation in diverse cell types.
Authors:
Deed, Richard W; Bianchi, S M; Atherton, Graham T; Johnston, D; Santibanez-Koref, Mauro F; Murphy, J J; Norton, John D
Abstract:
Transcription factors characterized by the presence of a helix-loop-helix (HLH) domain play a central role in the regulation of cell growth/differentiation and tumorigenesis. We report here the cDNA sequence of a human early-response gene, designated HLH 1R21, encoding a 15-kDa HLH protein that lacks a basic, DNA-binding domain and which by a number of criteria appears to be the human homologue of mouse HLH 462. Like its murine counterpart, HLH 1R21 protein functions as an Id (inhibitor of DNA binding) transcription factor by inhibiting the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer oligonucleotide in vitro. However HLH 1R21 does not inhibit the binding of HLH Max protein to a Max-binding oligonucleotide in vitro, indicating that it has limited promiscuity in its ability to antagonize the function of other HLH transcription factors. In addition, HLH 1R21 mRNA transcripts are regulated by phorbol ester treatment of a diverse range of human cell lines and, when overexpressed in mouse NIH3T3 cells, HLH 1R21 induces a morphologically transformed phenotype.
Affiliation:
CRC Department of Gene Regulation, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
An immediate early human gene encodes an Id-like helix-loop-helix protein and is regulated by protein kinase C activation in diverse cell types. 1993, 8 (3):599-607 Oncogene
Journal:
Oncogene
Issue Date:
Mar-1993
URI:
http://hdl.handle.net/10541/100233
PubMed ID:
8437843
Type:
Article
Language:
en
ISSN:
0950-9232
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorDeed, Richard Wen
dc.contributor.authorBianchi, S Men
dc.contributor.authorAtherton, Graham Ten
dc.contributor.authorJohnston, Den
dc.contributor.authorSantibanez-Koref, Mauro Fen
dc.contributor.authorMurphy, J Jen
dc.contributor.authorNorton, John Den
dc.date.accessioned2010-06-03T14:47:15Z-
dc.date.available2010-06-03T14:47:15Z-
dc.date.issued1993-03-
dc.identifier.citationAn immediate early human gene encodes an Id-like helix-loop-helix protein and is regulated by protein kinase C activation in diverse cell types. 1993, 8 (3):599-607 Oncogeneen
dc.identifier.issn0950-9232-
dc.identifier.pmid8437843-
dc.identifier.urihttp://hdl.handle.net/10541/100233-
dc.description.abstractTranscription factors characterized by the presence of a helix-loop-helix (HLH) domain play a central role in the regulation of cell growth/differentiation and tumorigenesis. We report here the cDNA sequence of a human early-response gene, designated HLH 1R21, encoding a 15-kDa HLH protein that lacks a basic, DNA-binding domain and which by a number of criteria appears to be the human homologue of mouse HLH 462. Like its murine counterpart, HLH 1R21 protein functions as an Id (inhibitor of DNA binding) transcription factor by inhibiting the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer oligonucleotide in vitro. However HLH 1R21 does not inhibit the binding of HLH Max protein to a Max-binding oligonucleotide in vitro, indicating that it has limited promiscuity in its ability to antagonize the function of other HLH transcription factors. In addition, HLH 1R21 mRNA transcripts are regulated by phorbol ester treatment of a diverse range of human cell lines and, when overexpressed in mouse NIH3T3 cells, HLH 1R21 induces a morphologically transformed phenotype.en
dc.language.isoenen
dc.subject.mesh3T3 Cells-
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshCell Transformation, Neoplastic-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshEnzyme Activation-
dc.subject.meshGene Expression Regulation-
dc.subject.meshGenome, Human-
dc.subject.meshHumans-
dc.subject.meshImmunoglobulin Idiotypes-
dc.subject.meshInhibitor of Differentiation Proteins-
dc.subject.meshMice-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshProtein Kinase C-
dc.subject.meshProteins-
dc.subject.meshSequence Homology, Amino Acid-
dc.subject.meshTetradecanoylphorbol Acetate-
dc.subject.meshTranscription Factors-
dc.titleAn immediate early human gene encodes an Id-like helix-loop-helix protein and is regulated by protein kinase C activation in diverse cell types.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Gene Regulation, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalOncogeneen

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