Haemopoietic stem cell development to neutrophils is associated with subcellular redistribution and differential expression of protein kinase C subspecies.

2.50
Hdl Handle:
http://hdl.handle.net/10541/100089
Title:
Haemopoietic stem cell development to neutrophils is associated with subcellular redistribution and differential expression of protein kinase C subspecies.
Authors:
Shearman, M S; Heyworth, Clare M; Dexter, T Michael; Haefner, B; Owen, P J; Whetton, Anthony D
Abstract:
Multipotential FDCP-Mix A4 (A4) cells can be induced either to self-renew or to differentiate and develop into mature neutrophils in liquid culture, depending on the haemopoietic growth factors with which they are cultured. When cultured in low concentrations of interleukin 3 (IL-3, 1 unit/ml)) plus Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) and Granulocyte-CSF (G-CSF), A4 cells proliferate with accompanying development to form cells which resemble mature, postmitotic neutrophils. The presence of high concentrations of IL-3 (100 units/ml) blocks the development of A4 cells even in the presence of GM-CSF plus G-CSF. A4 cell development to neutrophils is accompanied by major changes in the expression of protein kinase C (PKC) subspecies in these cells. The predominant subspecies present in multipotent A4 cells, as judged by direct chromatographic analysis, was the type III enzyme (alpha) subspecies, whereas in mature A4 cell neutrophils, the type II (beta I + beta II) enzymes were predominant. Phorbol esters added to immature A4 cells resulted in a proliferative response, but when added to postmitotic A4 cells resembling neutrophils they elicited a large increase in reactive oxygen intermediate production. This suggests that the type III (alpha) subspecies may mediate proliferative responses in stem cells, whilst the type II (beta I + beta II) enzymes are more important for the mature cell functions of postmitotic neutrophils. In cultures containing IL-3 (100 units/ml) both the type III, and also the type II subspecies were predominantly membrane-associated for prolonged periods (> 24 hours).(ABSTRACT TRUNCATED AT 250 WORDS)
Affiliation:
Department of Biochemistry, Kobe University School of Medicine, Japan.
Citation:
Haemopoietic stem cell development to neutrophils is associated with subcellular redistribution and differential expression of protein kinase C subspecies. 1993, 104 ( Pt 1):173-80 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
Jan-1993
URI:
http://hdl.handle.net/10541/100089
PubMed ID:
8449995
Type:
Article
Language:
en
ISSN:
0021-9533
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorShearman, M Sen
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorHaefner, Ben
dc.contributor.authorOwen, P Jen
dc.contributor.authorWhetton, Anthony Den
dc.date.accessioned2010-06-01T15:32:25Z-
dc.date.available2010-06-01T15:32:25Z-
dc.date.issued1993-01-
dc.identifier.citationHaemopoietic stem cell development to neutrophils is associated with subcellular redistribution and differential expression of protein kinase C subspecies. 1993, 104 ( Pt 1):173-80 J. Cell. Sci.en
dc.identifier.issn0021-9533-
dc.identifier.pmid8449995-
dc.identifier.urihttp://hdl.handle.net/10541/100089-
dc.description.abstractMultipotential FDCP-Mix A4 (A4) cells can be induced either to self-renew or to differentiate and develop into mature neutrophils in liquid culture, depending on the haemopoietic growth factors with which they are cultured. When cultured in low concentrations of interleukin 3 (IL-3, 1 unit/ml)) plus Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) and Granulocyte-CSF (G-CSF), A4 cells proliferate with accompanying development to form cells which resemble mature, postmitotic neutrophils. The presence of high concentrations of IL-3 (100 units/ml) blocks the development of A4 cells even in the presence of GM-CSF plus G-CSF. A4 cell development to neutrophils is accompanied by major changes in the expression of protein kinase C (PKC) subspecies in these cells. The predominant subspecies present in multipotent A4 cells, as judged by direct chromatographic analysis, was the type III enzyme (alpha) subspecies, whereas in mature A4 cell neutrophils, the type II (beta I + beta II) enzymes were predominant. Phorbol esters added to immature A4 cells resulted in a proliferative response, but when added to postmitotic A4 cells resembling neutrophils they elicited a large increase in reactive oxygen intermediate production. This suggests that the type III (alpha) subspecies may mediate proliferative responses in stem cells, whilst the type II (beta I + beta II) enzymes are more important for the mature cell functions of postmitotic neutrophils. In cultures containing IL-3 (100 units/ml) both the type III, and also the type II subspecies were predominantly membrane-associated for prolonged periods (> 24 hours).(ABSTRACT TRUNCATED AT 250 WORDS)en
dc.language.isoenen
dc.subjectHaematopoietic Cell Growth Factorsen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation-
dc.subject.meshCells, Cultured-
dc.subject.meshCytosol-
dc.subject.meshDNA Replication-
dc.subject.meshEnzyme Activation-
dc.subject.meshHematopoietic Cell Growth Factors-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshInterleukin-3-
dc.subject.meshIsoenzymes-
dc.subject.meshMembrane Proteins-
dc.subject.meshN-Formylmethionine Leucyl-Phenylalanine-
dc.subject.meshNeutrophils-
dc.subject.meshProtein Kinase C-
dc.subject.meshRespiratory Burst-
dc.subject.meshTetradecanoylphorbol Acetate-
dc.titleHaemopoietic stem cell development to neutrophils is associated with subcellular redistribution and differential expression of protein kinase C subspecies.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biochemistry, Kobe University School of Medicine, Japan.en
dc.identifier.journalJournal of Cell Scienceen
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