Expression of human papillomavirus type 16 E7 protein by recombinant baculovirus and use for the detection of E7 antibodies in sera from cervical carcinoma patients.

2.50
Hdl Handle:
http://hdl.handle.net/10541/100081
Title:
Expression of human papillomavirus type 16 E7 protein by recombinant baculovirus and use for the detection of E7 antibodies in sera from cervical carcinoma patients.
Authors:
Stacey, Simon N; Ghosh, Anna K; Bartholomew, Jennifer S; Tindle, R W; Stern, Peter L; Mackett, Mike; Arrand, John R
Abstract:
Although the presence of serum antibodies against the human papillomavirus type 16 (HPV-16) E7 protein has been linked with cervical cancer, currently available assays detect antibodies in only ca. 40% of carcinoma patients. The dependence of these serological assays on synthetic target antigens which present only linear epitopes may be a limiting factor. In order to produce a more realistic target antigen for use in serological assays, we have expressed the HPV-16 E7 protein in insect cells using a recombinant baculovirus vector. Two major E7 forms of ca. 18kDa and 16kDa were produced and characterised. The 16kDa component was shown to be truncated at the N-terminus. A radioimmunoprecipitation assay was developed for the detection of anti-E7 antibodies in human sera. This assay showed a marked increase in detection rate compared with a western blotting method based on bacterially derived E7 fusion proteins.
Affiliation:
Department of Molecular Biology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, England.
Citation:
Expression of human papillomavirus type 16 E7 protein by recombinant baculovirus and use for the detection of E7 antibodies in sera from cervical carcinoma patients. 1993, 40 (1):14-21 J. Med. Virol.
Journal:
Journal of Medical Virology
Issue Date:
May-1993
URI:
http://hdl.handle.net/10541/100081
PubMed ID:
7685808
Type:
Article
Language:
en
ISSN:
0146-6615
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorStacey, Simon Nen
dc.contributor.authorGhosh, Anna Ken
dc.contributor.authorBartholomew, Jennifer Sen
dc.contributor.authorTindle, R Wen
dc.contributor.authorStern, Peter Len
dc.contributor.authorMackett, Mikeen
dc.contributor.authorArrand, John Ren
dc.date.accessioned2010-06-01T15:09:27Z-
dc.date.available2010-06-01T15:09:27Z-
dc.date.issued1993-05-
dc.identifier.citationExpression of human papillomavirus type 16 E7 protein by recombinant baculovirus and use for the detection of E7 antibodies in sera from cervical carcinoma patients. 1993, 40 (1):14-21 J. Med. Virol.en
dc.identifier.issn0146-6615-
dc.identifier.pmid7685808-
dc.identifier.urihttp://hdl.handle.net/10541/100081-
dc.description.abstractAlthough the presence of serum antibodies against the human papillomavirus type 16 (HPV-16) E7 protein has been linked with cervical cancer, currently available assays detect antibodies in only ca. 40% of carcinoma patients. The dependence of these serological assays on synthetic target antigens which present only linear epitopes may be a limiting factor. In order to produce a more realistic target antigen for use in serological assays, we have expressed the HPV-16 E7 protein in insect cells using a recombinant baculovirus vector. Two major E7 forms of ca. 18kDa and 16kDa were produced and characterised. The 16kDa component was shown to be truncated at the N-terminus. A radioimmunoprecipitation assay was developed for the detection of anti-E7 antibodies in human sera. This assay showed a marked increase in detection rate compared with a western blotting method based on bacterially derived E7 fusion proteins.en
dc.language.isoenen
dc.subjectTumour Virus Infectionsen
dc.subjectUterine Cervical Canceren
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAntibodies, Viral-
dc.subject.meshBaculoviridae-
dc.subject.meshBase Sequence-
dc.subject.meshBlotting, Western-
dc.subject.meshEpitopes-
dc.subject.meshFalse Negative Reactions-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshOncogene Proteins, Viral-
dc.subject.meshOpen Reading Frames-
dc.subject.meshPapillomaviridae-
dc.subject.meshRadioimmunoprecipitation Assay-
dc.subject.meshRecombinant Proteins-
dc.subject.meshTumor Virus Infections-
dc.subject.meshUterine Cervical Neoplasms-
dc.titleExpression of human papillomavirus type 16 E7 protein by recombinant baculovirus and use for the detection of E7 antibodies in sera from cervical carcinoma patients.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Biology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, England.en
dc.identifier.journalJournal of Medical Virologyen

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